CARLIN: A Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression.

Autor:	Bowling S; Stem Cell Program, Boston Children's Hospital, Boston, MA, USA. sarah.bowling@stanford.edu.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA. sarah.bowling@stanford.edu.; Department of Developmental Biology, Stanford University School of Medicine, Stanford, United States. sarah.bowling@stanford.edu., Camargo FD; Stem Cell Program, Boston Children's Hospital, Boston, MA, USA.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2886, pp. 281-298.
DOI:	10.1007/978-1-0716-4310-5_14
Abstrakt:	The CRISPR-activated repair lineage tracing (CARLIN) mouse line uses DNA barcoding to enable high-resolution tracing of cell lineages in vivo (Bowling et al, Cell 181, 1410-1422.e27, 2020). CARLIN mice contain expressed barcodes that allow simultaneous interrogation of lineage and gene expression information from single cells. Furthermore, barcode editing is fully inducible, resulting in cell lineage labeling that can be performed at any time point in development or adulthood. This chapter details the protocols followed for maintaining CARLIN mice, inducing barcoding, and amplifying the CARLIN barcode from DNA, RNA, and single-cell RNA-sequencing libraries for next-generation sequencing. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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