Centrophilic retrotransposon integration via CENH3 chromatin in Arabidopsis.

Autor: Tsukahara S; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan. stsukaha@g.ecc.u-tokyo.ac.jp., Bousios A; School of Life Sciences, University of Sussex, Brighton, UK. ab35@sussex.ac.uk., Perez-Roman E; School of Life Sciences, University of Sussex, Brighton, UK., Yamaguchi S; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan., Leduque B; Institute of Plant Sciences Paris-Saclay (IPS2), Centre National de la Recherche Scientifique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université Evry, Université Paris-Saclay, Gif sur Yvette, France., Nakano A; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan., Naish M; Department of Plant Sciences, University of Cambridge, Cambridge, UK., Osakabe A; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan., Toyoda A; Center for Genetic Resource Information, National Institute of Genetics, Mishima, Japan., Ito H; Faculty of Science, Hokkaido University, Sapporo, Japan., Edera A; Institute of Plant Sciences Paris-Saclay (IPS2), Centre National de la Recherche Scientifique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université Evry, Université Paris-Saclay, Gif sur Yvette, France., Tominaga S; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan., Juliarni; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan., Kato K; Department of Integrated Genetics, National Institute of Genetics, Mishima, Japan., Oda S; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan., Inagaki S; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan., Lorković Z; Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna, Austria., Nagaki K; Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan., Berger F; Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna, Austria., Kawabe A; Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan., Quadrana L; Institute of Plant Sciences Paris-Saclay (IPS2), Centre National de la Recherche Scientifique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université Evry, Université Paris-Saclay, Gif sur Yvette, France., Henderson I; Department of Plant Sciences, University of Cambridge, Cambridge, UK., Kakutani T; Department of Biological Sciences, The University of Tokyo, Tokyo, Japan. tkak@bs.s.u-tokyo.ac.jp.
Jazyk: angličtina
Zdroj: Nature [Nature] 2025 Jan 01. Date of Electronic Publication: 2025 Jan 01.
DOI: 10.1038/s41586-024-08319-7
Abstrakt: In organisms ranging from vertebrates to plants, major components of centromeres are rapidly evolving repeat sequences, such as tandem repeats (TRs) and transposable elements (TEs), which harbour centromere-specific histone H3 (CENH3) 1,2 . Complete centromere structures recently determined in human and Arabidopsis suggest frequent integration and purging of retrotransposons within the TR regions of centromeres 3-5 . Despite the high impact of 'centrophilic' retrotransposons on the paradox of rapid centromere evolution, the mechanisms involved in centromere targeting remain poorly understood in any organism. Here we show that both Ty3 and Ty1 long terminal repeat retrotransposons rapidly turnover within the centromeric TRs of Arabidopsis species. We demonstrate that the Ty1/Copia element Tal1 (Transposon of Arabidopsis lyrata 1) integrates de novo into regions occupied by CENH3 in Arabidopsis thaliana, and that ectopic expansion of the CENH3 region results in spread of Tal1 integration regions. The integration spectra of chimeric TEs reveal the key structural variations responsible for contrasting chromatin-targeting specificities to centromeres versus gene-rich regions, which have recurrently converted during the evolution of these TEs. Our findings show the impact of centromeric chromatin on TE-mediated rapid centromere evolution, with relevance across eukaryotic genomes.
Competing Interests: Competing interests: The authors declare no competing interests.
(© 2025. The Author(s).)
Databáze: MEDLINE
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