Evaluation of the bone regenerative effect of glycogen synthase kinase 3 antagonist Tideglusib carried by different scaffolds on rat calvarial defects.

Autor: Çalık M; Department of Periodontology, Faculty of Dentistry, Marmara University, Istanbul 34854, Turkiye., Unal S; Genetic and Metabolic Diseases Research and Investigation Center, Marmara University, Istanbul, Turkiye. Electronic address: semra.unal@marmara.edu.tr., Alemdag B; Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkiye., Gündüz O; Center for Nanotechnology and Biomaterials Application and Research, Marmara University, Istanbul 34722, Turkiye; Department of Metallurgy and Material Engineering, Faculty of Technology, Marmara University, Istanbul 34722, Turkiye. Electronic address: oguzhan@marmara.edu.tr., Tekkeşin MS; Department of Oral Pathology, Faculty of Dentistry, Istanbul University, Istanbul, Turkiye; Department of Tumour Pathology, Institute of Oncology, Istanbul University, Istanbul, Turkiye. Electronic address: msoluk@istanbul.edu.tr., Ozen B; Department of Periodontology, Faculty of Dentistry, Marmara University, Istanbul 34854, Turkiye., Islek I; Department of Periodontology, Faculty of Dentistry, Marmara University, Istanbul 34854, Turkiye., Kuru L; Department of Periodontology, Faculty of Dentistry, Marmara University, Istanbul 34854, Turkiye. Electronic address: lkuru@marmara.edu.tr., Agrali OB; Department of Periodontology, Faculty of Dentistry, Marmara University, Istanbul 34854, Turkiye. Electronic address: omer.agrali@marmara.edu.tr.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec 30, pp. 139350. Date of Electronic Publication: 2024 Dec 30.
DOI: 10.1016/j.ijbiomac.2024.139350
Abstrakt: The aim was to explore the efficiency of Tideglusib in bone tissue healing by carrying it with different scaffolds on rat calvarial lesions. Twentyfour male Dawley rats were utilized. Two bone defects of 5 mm in diameter were formed (n = 8). Groups constituted negative control, collagen sponge + Tideglusib (CT), bacterial cellulose carrier (BC), bacterial cellulose carrier + Tideglusib (BC + T), PCL/Gel nanocarrier (Nano) and PCL/Gel + Tideglusib (Nano+T). After four week, histomorphometric and immunohistochemistry investigations were performed. Pairwise comparisons by means of the new bone formation (NBF) effect of Tideglusib demonstrated a significant difference between the control and the Nano+T groups solely (p < 0.05). BC group demonstrated reduced NBF in comparison to the CT group (p < 0.05), Nano group (p < 0.01) and Nano+T group (p < 0.01). Similarly, the BC + T group exhibited a diminished rate of NBF in comparison to both the Nano (p < 0.01) and Nano+T groups (p < 0.01). Type I collagen expression decreased in the BC group (p < 0.05) and BC + T group (p < 0.05) relative to the control. Axin2 expression was increased in the Nano+T group (p < 0.05) compared to the control. Within the limits, Tideglusib delivered with a nanocarrier containing PCL/Gel may have favorable impact on bone regeneration. However, the impact may vary with different carrier.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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