Exploration the Effects of Mesenchymal Stem Cells and Olive Leaf Extract on Physiological and Histopathological Changes on the Kidney of Diabetes Rats.

Autor:	AbdRabou MA; Olive Research Center, Department of Biology, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia, mababdraboh@ju.edu.sa., Atwa A; Zoology Department, Faculty of Science (Boys), Al-Azhar University, Cairo 11884, Egypt., Alrayes ZR; Olive Research Center, Department of Biology, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia., Massoud D; Olive Research Center, Department of Biology, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia., Alghamdi FA; Biology Department, College of Science, University of Jeddah, Jeddah 23218, Saudi Arabia., Asran AM; Olive research center, Common First Year Dean's Office, Jouf University, El Jouf, Saudi Arabia., Germoush MO; Olive Research Center, Department of Biology, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia., Alharbi HM, Alruwaili HK; Olive Research Center, Department of Biology, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia., Faddladdeen KAJ; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, 13225 Riyadh, Saudi Arabia., Mehany ABM; Zoology Department, Faculty of Science (Boys), Al-Azhar University, Cairo 11884, Egypt.
Jazyk:	angličtina
Zdroj:	Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2024 Dec 28; Vol. 58 (6), pp. 807-822.
DOI:	10.33594/000000748
Abstrakt:	Background/aims: Gestational Diabetes Mellitus (GDM) is a common complication during pregnancy, defined as diabetes diagnosed in the second or third trimester, often asymptomatic. This study investigates the therapeutic potential of olive leaf extracts and stem cells in mitigating GDM-induced complications, particularly focusing on renal function, oxidative stress, and pancreatic cell regeneration. Methods: Measurements were made in gravid female rats with or without intraperitoneal administration of Streptozotocin (35 mg/kg body weight). Biochemical analyses were conducted to evaluate renal function markers (urea, uric acid, creatinine) and oxidative stress parameters (malondialdehyde, glutathione, and superoxide dismutase levels). Histopathological and immunohistopathological evaluations of kidney tissues were performed using hematoxylin and eosin staining and specific markers (p53, Insulin, and PCNA) to assess cellular changes. Results: The diabetic group exhibited significantly elevated levels of urea, uric acid, and creatinine (p<0.01) compared to the control group. Treatment with stem cells and olive leaf extracts significantly reduced these levels. Malondialdehyde levels were elevated in the diabetic group (p<0.01) but showed marked improvement in the treatment groups. Additionally, glutathione and superoxide dismutase activities were diminished in the diabetic rats (p<0.05) but increased following treatment. Histopathological and immunohistopathological analyses revealed cellular regeneration and improved tissue morphology in the treatment groups compared to the diabetic group. Conclusion: Stem cells and olive leaf extracts exhibit significant therapeutic potential in ameliorating renal dysfunction, oxidative stress, and tissue damage associated with GDM, highlighting their role in enhancing pancreatic cell regeneration. Competing Interests: All authors declare that they have no conflicts of interest. (© Copyright by the Author(s). Published by Cell Physiol Biochem Press.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu