Dopamine D2 receptors in the bed nucleus of the stria terminalis modulate alcohol-related behaviors.

Autor:	Pati D; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Taxier LR; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Xia M; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Lee SI; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Conley SY; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.; Curriculum of Neuroscience, University of North Carolina School of Medicine, Chapel Hill, NC, USA., Sides T; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Boyt KM; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Hunker AC; Department of Pharmacology, University of Washington, Seattle, WA, USA., Zweifel LS; Department of Pharmacology, University of Washington, Seattle, WA, USA.; Department of Psychiatry & Behavioral Sciences, University of Washington, Seattle, WA, USA., Kash TL; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Jazyk:	angličtina
Zdroj:	Addiction neuroscience [Addict Neurosci] 2024 Jun; Vol. 11. Date of Electronic Publication: 2024 May 08.
DOI:	10.1016/j.addicn.2024.100157
Abstrakt:	Dysregulation of the dopamine (DA) system is a hallmark of substance use disorders, including alcohol use disorder (AUD). Of the DA receptor subtypes, the DA D2 receptors (D2Rs) play a key role in the reinforcing effects of alcohol. D2Rs are expressed in numerous brain regions associated with the regulation of appetitive behaviors. One such region is the bed nucleus of the stria terminalis (BNST), which has been linked to the development and maintenance of AUD. Recently, we identified alcohol withdrawal-related neuroadaptations in the periaqueductal gray/dorsal raphe to BNST DA circuit in male mice. However, the role of D2R-expressing BNST neurons in voluntary alcohol consumption is not well characterized. In this study, we used a CRISPR-Cas9-based viral approach, to selectively reduce the expression of D2Rs in BNST GABA neurons (BNST vgat Drd2 ) and interrogated the impact on alcohol-related behaviors. In male mice, reduced BNST vgat Drd2 expression potentiated the stimulatory effects of alcohol and increased voluntary consumption of 20% w/v alcohol in a two-bottle choice intermittent access paradigm. This effect was not specific to alcohol, as BNST vgat Drd2 knockdown also increased sucrose intake in male mice. Interestingly, reduction in BNST vgat Drd2 expression in female mice did not alter alcohol-related behaviors but lowered the threshold for mechanical pain sensitivity. Collectively, our findings suggest a role for postsynaptic BNST D2Rs in the modulation of sex-specific behavioral responses to alcohol and sucrose. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Databáze:	MEDLINE
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