Characterisation of Aequorivita ciconiae sp. nov., isolated from oriental stork, Ciconia boyciana.

Autor: Han JE; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea., Kang S; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea., Lee SY; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea., Bae JW; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea. baejw@khu.ac.kr.; Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea. baejw@khu.ac.kr.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2024 Dec 31; Vol. 118 (2), pp. 47. Date of Electronic Publication: 2024 Dec 31.
DOI: 10.1007/s10482-024-02056-7
Abstrakt: A single novel bacterial strain designated as H23M31 T was isolated from the faecal sample of oriental stork (Ciconia boyciana) that inhabits the Republic of Korea. It was a rod-shaped, facultative anaerobic, Gram-negative, and non-motile strain. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that it branched from Aequorivita within Flavobacteriaceae. It was most closely related to A. capsosiphonis DSM 23843  T , which shared the sequence similarity of 96.36%. The strain exhibited optimal growth at pH 7.0 in a marine broth medium with 1% NaCl incubated at 30 °C. Chemotaxonomic characteristics indicated that the predominant cellular fatty acids were iso-C 15:0 (24.4%), iso-C 17:0 3-OH (15.9%), and anteiso-C 15:0 (13.9%). The polar lipid of the strain contained phosphatidylcholine (PC), phosphatidylglycerol (PG), and diphosphatidylglycerol (DPG). The major isoprenoid quinone was menaquinone 6 (MK-6), which was identical with that of a closely related Aequorivita species. The genomic G + C contents of the strain was 38.25 mol%. Average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between the novel isolate and A. viscosa CGMCC 1.11023  T were 75.83%, 80.34% and 20.50%, respectively. Phylogenetic analyses revealed the evolutionary relationships of the strain, demonstrating that the strain clusters with other Aequorivita species. Pan-genome analyses and genome comparisons indicated that, unlike other environmentally isolated species, it possesses unique genes that enhance its ability to colonise the harsh animal gut environment. Taxonomic characterisation suggested that strain H23M31 T represents a novel Aequorivita species, and the proposed name is Aequorivita ciconiae sp. nov. The type strain of A. ciconiae is H23M31 T (= KCTC 62809  T  = JCM 33229  T ).
Competing Interests: Declarations. Conflicts of interest: The authors declare that they have no conflict of interest. Ethical approval: The sampling and experimental procedures implemented in this study were approved by the Institutional Animal Care and Use Committee of Kyung Hee University (Permit number: KHUASP(SE)-18–048).
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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