Microscopic changes in the multifidus muscle in people with low back pain associated with lumbar disc herniation.

Autor: Purushotham S; Centre of Precision Rehabilitation for Spinal Pain (CPR Spine), School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, B15 2TT, UK.; Department of Biomedical Sciences, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK., Hodson N; Department of Sport and Exercise Sciences, Institute of Sport, Manchester Metropolitan University, Manchester, M15 6BH, UK., Greig C; School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, B15 2TT, UK.; MRC-Versus Arthritis Centre for Musculoskeletal Ageing and Health, University of Birmingham, Birmingham, B15 2TT, UK.; National Institute for Health Research, Birmingham Biomedical Research Centre at University Hospitals Birmingham NHS Foundation Trust, Birmingham, B15 2TT, UK., Gardner A; The Royal Orthopaedic Hospital NHS Foundation Trust, Northfield, Birmingham, B31 2AP, UK.; College of Health and Life Sciences, Aston University, Birmingham, B4 7ET, UK., Falla D; Centre of Precision Rehabilitation for Spinal Pain (CPR Spine), School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, B15 2TT, UK. d.falla@bham.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Dec 30; Vol. 14 (1), pp. 31927. Date of Electronic Publication: 2024 Dec 30.
DOI: 10.1038/s41598-024-83373-9
Abstrakt: Lumbar disc herniation (LDH) is a common degenerative condition causing low back pain (LBP) due to nerve compression. Previous studies show conflicting findings regarding the multifidus (MF) muscle's microscopic changes in LDH patients. So, this study aimed to compare the affected MF to the adjacent MF on the ipsilateral and contralateral sides in LDH patients and examined correlations with clinical features of LBP. Four muscle biopsies were collected from each of 30 surgical participants. Immunohistochemistry was performed on tissue sections and imaged with an epifluorescence microscope. Data was analysed using a two-way ANOVA for muscle fibre cross-sectional area, perimeter, diameter, and composition, while pathological fibres were analysed using a one-way ANOVA. Pearson's correlation was employed to examine MF microscopy associations with clinical features. Results revealed no significant differences between the affected MF and MF from other sites, though significantly more pathological fibres were present in the affected MF (p < 0.05). A weak but significant negative correlation was found between type I fibres and LBP clinical features, though no such correlations were observed for type IIA fibres. In conclusion, LDH primarily impacts the pathological status of the MF rather than fibre phenotype or size, and severity of clinical features is associated with the size of type I fibres.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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