Mapping quantitative trait loci regions associated with Marek's disease on chicken autosomes by means of selective DNA pooling.

Autor: Lipkin E; Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem, 91904, Israel. ehud.lipkin@mail.huji.ac.il., Smith J; The Roslin Institute and Royal (Dick) School of Veterinary Studies R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK. jacqueline.smith@roslin.ed.ac.uk., Soller M; Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem, 91904, Israel., Burt DW; The Roslin Institute and Royal (Dick) School of Veterinary Studies R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Fulton JE; Hy-Line International, 2583 240th St, PO Box 310, Dallas Center, 50063, IA, USA.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Dec 30; Vol. 14 (1), pp. 31896. Date of Electronic Publication: 2024 Dec 30.
DOI: 10.1038/s41598-024-83356-w
Abstrakt: Marek's Disease (MD), which can result in neurological damage and tumour formation, has large effects on the economy and animal welfare of the poultry industry worldwide. Previously, we mapped autosomal MD QTL regions (QTLRs) by individual genotyping of an F 6 population from a full-sib advanced intercross line. We further mapped MD QTLRs on the chicken Z chromosome (GGZ) using the same F 6 population, and by selective DNA pooling (SDP) of 8 elite egg production lines. Here we used SDP of the same pools used on GGZ to map autosomal MD QTLRs. Thirty-seven QTLRs were found. Seven of the QTLRs were tested by all sires from the same 8 lines, individually genotyped for QTLR markers. Five of the tested QTLRs were confirmed. Linkage disequilibrium (LD) was calculated for all QTLR markers on the same chromosome, and complex LD blocks were found. Distribution of P and LD values were used to assess the QTLR causative elements. Allele substitution effects were calculated based on both pooled SNP microarray genotypes, and individual genotypes of QTLRs markers. Substantial allele effect and contribution to the phenotypic and genotypic variation were obtained. The results explain part of the MD response, and provide targets for mitigating MD.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethics approval: Institutional Review Board Statement: The animal study protocol was approved by Hy-Line International Institutional Animal Care and Use Committee guidelines. Hy-Line do not have specific project identification codes. All of these samples had been collected in the past and stored frozen until use. At the termination of the study animals were humanely euthanized under the approval of the Hy-Line IACUC and supervision of licensed veterinarians.
(© 2024. The Author(s).)
Databáze: MEDLINE
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