Antiviral Properties of Essential Oil Mixture: Modulation of E7 and E2 Protein Pathways in Human Papillomavirus (HPV) Infection.

Autor: Uner B; Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul Kent University, Istanbul 34406, Türkiye; Department of Pharmaceutical and Administrative Sciences, University of Health Science and Pharmacy in St. Louis, St. Louis, MO, 63110, USA; Department of Anesthesiology, Center for Clinical Pharmacology, Washington University School of Medicine in St. Louis, St. Louis, MO, 63110, USA; MecNano Technologies, Cube Incubation, Teknopark Istanbul, Istanbul 34906, Türkiye. Electronic address: b.urcu.uner@uhsp.edu., Guler E; MecNano Technologies, Cube Incubation, Teknopark Istanbul, Istanbul 34906, Türkiye; Department of Pharmacology, Faculty of Pharmacy, Istanbul Kent University, Istanbul 34406, Türkiye; UCL Division of Surgery and Interventional Sciences, Rowland Hill Street, NW3 2PF London, UK. Electronic address: ece.guler@kent.edu.tr., Vicir ME; Art de Huile, Teknopol Istanbul, Istanbul 34930, Türkiye. Electronic address: mustafavicir@artdehuile.com., Kayhan H; Art de Huile, Teknopol Istanbul, Istanbul 34930, Türkiye. Electronic address: hulyakayhan@artdehuile.com., Atsu N; Department of Pharmacology, Faculty of Pharmacy, Istanbul Kent University, Istanbul 34406, Türkiye. Electronic address: mehmetnecmettin.atsu@kent.edu.tr., Kalaskar D; UCL Division of Surgery and Interventional Sciences, Rowland Hill Street, NW3 2PF London, UK. Electronic address: d.kalaskar@ucl.ac.uk., Cam ME; MecNano Technologies, Cube Incubation, Teknopark Istanbul, Istanbul 34906, Türkiye; Department of Pharmacology, Faculty of Pharmacy, Istanbul Kent University, Istanbul 34406, Türkiye; UCL Division of Surgery and Interventional Sciences, Rowland Hill Street, NW3 2PF London, UK; Art de Huile, Teknopol Istanbul, Istanbul 34930, Türkiye; Biomedical Engineering Department, University of Aveiro, Aveiro 3810-193, Portugal. Electronic address: muhammet.cam@ucl.ac.uk.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2024 Dec 28, pp. 119289. Date of Electronic Publication: 2024 Dec 28.
DOI: 10.1016/j.jep.2024.119289
Abstrakt: Ethnopharmacological Relevance: Clove is used in Indian and Chinese traditional medicine for viral diseases. Palmarosa essential oils have been traditionally used in India and Southeast Asia since ancient times and have made considerable use of them. In New Caledonia, niaouli oil is used in aromatherapy and pharmaceutical formulations to treat pain and viral diseases. Since ancient times, the South Pacific region has used tamanu oil as a traditional medicine to treat a wide range of skin conditions.
Aim of the Study: This study investigates the antiviral properties of essential oils (EOs) from Eugenia aromaticum (clove oil, CL-R030424005 (CL)), Cymbopogon martinii (palmarosa oil, PA-R040923008 (PA)), Melaleuca viridiflora (niaouli oil, NI-R290124038 (NI)), and Calophyllum inophyllum (tamanu oil, TA-F140224029 (TA)), and their mixture against human papillomavirus (HPV) infection.
Materials and Methods: A D-optimal mixture design is used to determine the most effective EO combinations and evaluate their antiviral efficacy through IC 50 values. The EOs were tested for their ability to inhibit HPV-related oncogenes (L1, L2, E1, E2, E6, and E7) in HPV-infected cells with ELISA, qPCR, and Western blot analyses.
Results: and discussion: The optimal mixture (31.5% CL, 31.5% PA, and 37% NI) demonstrated significant antiviral activity, reducing viral replication and protein expression in HPV-infected cells. Ex-vivo permeation studies showed higher permeation rates in healthy tissues compared to infected ones, indicating the oils' potential in targeted drug delivery. Additionally, cytotoxicity assessments confirmed the safety of the EOs at effective concentrations in HPVCs, DoTc2, and HEKa cells. Molecular docking studies further elucidated the interactions between EO components and HPV proteins, supporting their antiviral mechanisms.
Conclusion: These findings suggest that EOs, particularly in optimized combinations, offer a promising natural supportive treatment for managing HPV infections, warranting further in vivo animal tests and clinical trials.
Competing Interests: Declaration of Competing Interest The authors have no competing interests.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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