Virus neutralization test for confirmation of ELISA-positive SARS-CoV-2 antibodies in dogs.

Autor:	Vujin D; Pasteur Institute of Novi Sad, Hajduk Veljkova 1, 21101 Novi Sad, Serbia., Knežević A; University of Belgrade, Faculty of Medicine, Institute of Microbiology, Pasterova 4, 11000 Belgrade, Serbia., Vračar V; University of Novi Sad, Faculty for Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia., Petrović D; University of Novi Sad, Faculty of Education in Sombor, Podgorička 4, 25000 Sombor, Serbia., Kozoderović G; University of Novi Sad, Faculty of Education in Sombor, Podgorička 4, 25000 Sombor, Serbia., Spasojević Kosić L; University of Novi Sad, Faculty for Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia., Lalošević V; University of Novi Sad, Faculty for Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia., Lalošević D; Pasteur Institute of Novi Sad, Hajduk Veljkova 1, 21101 Novi Sad, Serbia.
Jazyk:	angličtina
Zdroj:	Polish journal of veterinary sciences [Pol J Vet Sci] 2024 Dec; Vol. 27 (4), pp. 559-565.
DOI:	10.24425/pjvs.2024.152946
Abstrakt:	Constant antigenic changes, new variants and easy transmission of SARS-CoV-2 virus should acquire greater zoonotic attention and need to remain alert. In this retrospective study the aim was to analyze seropositivity to SARS-CoV-2 in dogs by commercial ELISA. The Virus neutralization test (VNT) was modified for the purpose of confirmation of SARS-CoV-2 antibodies in ELISA-positive dog sera. The sera were collected from 204 dogs from different veterinary clinics across Vojvodina Province, Serbia, during COVID-19 pandemic. For the screening of antibodies a commercial double multi-species antigen ELISA was used, followed by the VNT modified with SARS-CoV-2 as a confirmatory test. VNT was modified as "one step" test using local isolate of SARS-CoV-2 and the results were checked by cytopathic effect in cell culture on the 96-well microtiter plate. Obtained data have shown that 9 out of 204 dogs were positive by ELISA (4.4%), while 2 (0.97%) sera were doubtful. VNT confirmed 9 positive dogs, but 2 doubtful samples were negative, exhibiting the seroconversion to SARS-CoV-2 in 4.4% dogs from Vojvodina region during pandemic of COVID-19. VNT with SARS-CoV-2 helped to elucidate ELISA ambiguous results. The occurrence of antibodies to SARS-CoV-2 in dogs in this study during COVID-19 pandemic suggested the possibility of viral transmission to dogs, implicating the potential for zoonotic transmission. This was the first research on seroconversion to SARS-CoV-2 in dogs from the Province of Vojvodina, the northernmost part of Serbia. (© 2024 The Authors. This is an open access article under the CC BY-NC-ND 4.0 license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en), which allows re-users to copy and distribute the material in any medium or format in unadapted form and for noncommercial purposes, and only so long as attribution is given to the creator.)
Databáze:	MEDLINE
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