Single-cell transcriptome analysis of stem cells from human exfoliated deciduous teeth investigating functional heterogeneity in immunomodulation.

Autor: Li Y; Department of Stomatology, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, China. leeyin78@163.com.; Beijing Engineering Research Center of Immunocellular Therapy, Beijing, China. leeyin78@163.com., Song G; Beijing Engineering Research Center of Immunocellular Therapy, Beijing, China., Jiang Y; Biomedical Innovation Center, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.; Beijing Key Laboratory for Therapeutic Cancer Vaccines, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China., Zhao H; Department of Liver Surgery, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Beijing, China., Zhu Y; School of Pharmacy, Human Phenome Institute, Fudan University, Shanghai, 201203, China.; State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau, China., Song S; Beijing Engineering Research Center of Immunocellular Therapy, Beijing, China., Wang L; Beijing Engineering Research Center of Immunocellular Therapy, Beijing, China., Wu X; Biomedical Innovation Center, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China. wuxy@mail.ccmu.edu.cn.; Beijing Key Laboratory for Therapeutic Cancer Vaccines, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China. wuxy@mail.ccmu.edu.cn.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Dec 28; Vol. 14 (1), pp. 31279. Date of Electronic Publication: 2024 Dec 28.
DOI: 10.1038/s41598-024-82734-8
Abstrakt: Mesenchymal stem cells (MSCs) have been widely used in the treatment of various inflammatory diseases. The inadequate understanding of MSCs and their heterogeneity can impact the immune environment, which may be the cause of the good outcomes of MSCs-based therapy that cannot always be achieved. Recently, stem cells from human exfoliated deciduous teeth (SHED) showed great potential in inflammatory and autoimmune diseases due to their immature properties compared with MSCs. In our study, single-cell RNA sequencing (scRNA-seq) revealed that SHED in a low differentiation state (S7) exhibited the powerful ability to recruit multiple immune cells. In contrast, SHED in a relatively high differentiation state (S1) may hold a solid ability to secret many factors with paracrine signaling capacity. The analysis result shows that SHED has more robust immunomodulatory properties than human bone marrow-derived mesenchymal stem cells (hBMSCs) or human umbilical cord-derived mesenchymal stem cells (hUCMSCs). When co-cultured with PBMCs, SHED can enhance the proliferation of Treg and down-regulate TNF-α in vitro. SHED may have some advantages in the treatment of inflammatory and autoimmune diseases.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethics approval and consent to participate: Research involves the use of human data and tissue. Informed consent has been obtained from donor and his parent.
(© 2024. The Author(s).)
Databáze: MEDLINE
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