Isolation and characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis in North West Cameroon: public health implications.

Autor: Esemu SN; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Box 63, Buea, Cameroon. esemu2003@yahoo.co.uk.; Laboratory for Emerging Infectious Diseases, University of Buea, Box 63, Buea, Cameroon. esemu2003@yahoo.co.uk., Nya'Nying SF; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Box 63, Buea, Cameroon., Ndip LM; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Box 63, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Box 63, Buea, Cameroon., Bessong PO; South African Medical Research Council - University of Venda Antimicrobial Resistance and Global Health Research Unit, Thohoyandou, 0950, South Africa.; Center for Global Health Equity, University of Virginia, Charlottesville, USA., Tanih NF; Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Buea, Box 63, Buea, Cameroon., Smith SI; Department of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Lagos, Nigeria., Ndip RN; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Box 63, Buea, Cameroon.; Laboratory for Emerging Infectious Diseases, University of Buea, Box 63, Buea, Cameroon.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2024 Dec 27; Vol. 17 (1), pp. 389. Date of Electronic Publication: 2024 Dec 27.
DOI: 10.1186/s13104-024-07073-3
Abstrakt: Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic pathogen that poses a serious threat to veterinary and public health worldwide. We investigated mastitis milk samples for contamination with MRSA and also characterized the MRSA isolates by investigating antimicrobial resistance and virulence factors.
Result: We confirmed MRSA in 69 of 201 (34.3%) S. aureus isolates recovered from a total of 300 samples. Of the 69 MRSA, 19 (27.5%) were from subclinical cases, while 50 (72.5%) were from clinical cases. The MRSA showed high resistance to penicillin (100%), ampicillin (100%), trimethoprim (69.6%), and tetracycline (69.6%) while susceptibility was observed for gentamicin (100%), vancomycin (95.7%), and ciprofloxacin (91.3%). Most isolates (65.2%, 45/69) were multidrug resistant. Thirteen antibiotypes (A 1 -A 13 ) were identified and the most prevalent was A 8 (TMP R E R TET R AMP R P R) ). All MRSA produced haemolysins, caseinase, and coagulase. Lipase, gelatinase and lecithinase were found in 97.1%, 94.2% and 91.3% of isolates respectively. Genotyping revealed coa (100%) and spa (68.1%) genes. We recommend educating dairy farmers on the public health implications of consuming unpasteurized raw milk and the implementation of proper hygiene practices in dairy farms.
Competing Interests: Declarations. Ethical approval and consent to participate: This study was reviewed and approved (Ref. No. MINEPIA/DREPIA/NW/40/716/2019) by the Regional Delegation of Livestock, Fisheries and Animal Industries, a subunit of the country’s government institution that oversees livestock production and animal health for the North West Region of Cameroon. Collection of the milk samples was done after obtaining informed verbal consent from farm owners. All samples were collected by a trained veterinarian. No invasive procedures were involved, and very little pain was inflicted on the animals during the sample collection process. This study did not involve endangered or protected species and did not use animals for experiments. Animal ethics declaration: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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