Detection of methicillin resistance of Staphylococcus aureus in vitreous humor using MALDI-TOF MS and Fc-MBL@Fe 3 O 4 enrichment.

Autor: Ren J; Clinical Laboratory, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China., Gao W; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China., Yu M; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China., Liu C; Clinical Laboratory, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China., Ding C; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China., Li S; Clinical Laboratory, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China. lishengjie6363020@163.com., Yu S; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China. yushaoning@nbu.edu.cn., Cao W; Clinical Laboratory, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Dec 28; Vol. 14 (1), pp. 30757. Date of Electronic Publication: 2024 Dec 28.
DOI: 10.1038/s41598-024-80715-5
Abstrakt: Endophthalmitis is a serious infectious eye disease that causes permanent vision loss. This study developed a method for rapid identification and drug resistance analysis of pathogens in vitreous humor. After short-term rapid culture, 30 Staphylococcus aureus isolates were enriched and purified from the vitreous humor using Fc-MBL@Fe 3 O 4 , and then identified by MALDI-TOF MS. The bacterial solution was adjusted to 10 6 CFU/mL and mixed with CAMHB containing cefoxitin (4 µg/mL) at the same volume. After culture, it was enriched by Fc-MBL@Fe 3 O 4 and identified by MALDI-TOF MS. MRSA was judged according to whether the bacteria could successfully be identified. The enrichment efficiency of Fc-MBL@Fe 3 O 4 for S. aureus in CAMHB was 88.1%. The detection rate of S. aureus reached 100% after 8 h of vitreous humor culture. The best test performance was achieved with Fc-MBL@Fe 3 O 4 enrichment after a 3 h incubation. At this time point, 96.7% validity, 100% sensitivity, and 100% specificity were achieved. Thus, the identification and drug resistance analysis of S. aureus (51-110 CFU) in vitreous humor was completed within 11 h. This study provides a new method for rapid clinical diagnosis of endophthalmitis and precise treatment with antibiotics.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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