Daphnetin may protect from SARS-CoV-2 infection by reducing ACE2.

Autor: Yang QW; School of Life Sciences, Shanghai University, Shanghai, 200444, China.; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China., Yue CL; School of Life Sciences, Shanghai University, Shanghai, 200444, China.; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China., Chen M; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China.; Anhui Province Key Laboratory of Basic Research and Transformation of Age- related Diseases, Wannan Medical College, Wuhu, 241002, China., Ling YY; Department of Chemistry, Wannan Medical College, Wuhu, 241002, China., Dong Q; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China., Zhou YX; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China.; Anhui Province Key Laboratory of Basic Research and Transformation of Age- related Diseases, Wannan Medical College, Wuhu, 241002, China., Cao Y; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China.; Anhui Province Key Laboratory of Basic Research and Transformation of Age- related Diseases, Wannan Medical College, Wuhu, 241002, China., Ding YX; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China., Zhao X; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China., Huang H; School of Life Sciences, Shanghai University, Shanghai, 200444, China., Zhang ZH; Department of Laboratory Medicine, Changzheng Hospital, Naval Medical University, Shanghai, 200003, China. zhaohuanpost2016@163.com., Hu L; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China. huleiup@wnmc.edu.cn.; Anhui Province Key Laboratory of Basic Research and Transformation of Age- related Diseases, Wannan Medical College, Wuhu, 241002, China. huleiup@wnmc.edu.cn., Xu XH; School of Preclinical Medicine, Wannan Medical College, Wuhu, 241002, China. xhxu@wnmc.edu.cn.; Anhui Province Key Laboratory of Basic Research and Transformation of Age- related Diseases, Wannan Medical College, Wuhu, 241002, China. xhxu@wnmc.edu.cn.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Dec 28; Vol. 14 (1), pp. 30682. Date of Electronic Publication: 2024 Dec 28.
DOI: 10.1038/s41598-024-79734-z
Abstrakt: To combat the SARS-CoV-2 pandemic, innovative prevention strategies are needed, including reducing ACE2 expression on respiratory cells. This study screened approved drugs in China for their ability to downregulate ACE2. Daphnetin (DAP) was found to significantly reduce ACE2 mRNA and protein levels in PC9 cells. DAP exerts its inhibitory effects on ACE2 expression by targeting HIF-1α and JAK2, thereby impeding the transcription of the ACE2 gene. The SARS-CoV-2 pseudovirus infection assay confirmed that DAP-treated PC9 cells exhibited decreased susceptibility to viral infection. At therapeutic doses, DAP effectively lowers ACE2 expression in the respiratory systems of mice and humans. This suggests that DAP, already approved for other conditions, could be a new preventive measure against SARS-CoV-2, offering a cost-effective and accessible way to reduce SARS-CoV-2 spread.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Clinical ethics statement: Our study was conducted in accordance with the principles of the Declaration of Helsinki and was approved by the Medical Ethics Committee of Wannan Medical College to advertise for volunteers among Wannan Medical College teachers and students (Ref. No. 2023 − 205). The study was registered in China Clinical Research Registration Center, the registration number is ChiCTR2300077554. The registration date is 13/11/2023. Animal ethics statement: Animal experiments conducted in accordance with the regulations on the management of experimental animals issued by the State Commission for Science and Technology and the implementing rules on the management of medical experimental animals issued by the Ministry of Health of China, with the approval of the Laboratory Animal Welfare and Ethics Committee of Wannan Medical College (WNMC-AWE-2023459). All experimental procedures were done in compliance with the ARRIVE guidelines (PLoS Bio 8(6), e1000412,2010).
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje