Extraordinary SERS on nanostructured self-assembled silver films due to preliminary random nano-profiling of substrate and ultrasonic exposure of analyte during the deposition.

Autor: Vereshchagin KA; Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow 119991, Russia. Electronic address: stinkapella@mail.ru., Slipchenko EA; Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia., Boginskaya IA; Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia., Zvyagina JY; Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia., Afanasev KN; Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia., Dikikh DA; Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region 141700, Russia., Sedova MV; Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia., Ryzhikov IA; Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia; FMN Laboratory, Bauman Moscow State Technical University, Moscow 105005, Russia., Merzlikin AM; Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia; Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region 141700, Russia.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Dec 18; Vol. 329, pp. 125629. Date of Electronic Publication: 2024 Dec 18.
DOI: 10.1016/j.saa.2024.125629
Abstrakt: In this study, we have investigated the surface-enhanced Raman scattering (SERS) spectra of myoglobin on silver substrates with different morphology. The aim was to determine the optimal parameters of analyte and substrate preparation for obtaining of high-amplitude SERS spectra of proteins. It is shown that not only the morphology of the silver film, but also the method of analyte molecules deposition on the SERS substrate plays an important role. Glass substrates were pretreated several stages in organic solvents before silver deposition. It is shown that pretreatment of the glass surfaces lead to a decrease in the silver SERS film roughness. Myoglobin was applied to the SERS substrates in the form of an aqueous solution and then it was precipitated under ultrasonic treatment at frequencies of 580 kHz and 47 kHz. SERS spectra were recorded at randomly selected locations of the dried precipitate using excitation wavelengths of 785 nm and 532 nm. Unusual form myoglobin SERS spectra were observed, in which only certain part of the spectrum demonstrated the additional enhancement in comparison to the overall spectrum. We called such spectra extraordinary ones. In the extraordinary myoglobin SERS spectra, the amplitude of the vibrational band at 996 cm -1 is approximately five times more intense compared to other strong vibrational bands. And the ratio of amplitudes of vibrational band in extraordinary SESR spectra increased by more than an order of magnitude compared to the same ratio for amorphous myoglobin. The article discusses the mechanisms leading to the appearance of extraordinary SERS spectra.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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