Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis.

Autor:	Ali MA; Faculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Fayoum University, Fayoum, Egypt.; Department of Biomedical Sciences, College of Medicine, King Faisal University, Alhasa, Saudi Arabia., Shaker OG; Faculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Cairo University, Cairo, Egypt., Ezzat EM; Faculty of Medicine, Department of Internal Medicine, Fayoum University, Fayoum, Egypt., Ali ESG; Faculty of Medicine, Department of Medical Microbiology and Immunology, Fayoum University, Fayoum, Egypt., Aboelor MI; Faculty of Medicine, Department of Radio-Diagnosis, Fayoum University, Fayoum, Egypt., Ahmed MI; Faculty of Medicine, Department of Chest Disease, Fayoum University, Fayoum, Egypt., Hassan EA; Faculty of Medicine, Department of Tropical Medicine, Fayoum University, Fayoum, Egypt., Ali DY; Faculty of Medicine, Department of Clinical and Chemical Pathology, Fayoum University, Fayoum, Egypt., Ahmed HM; Faculty of Medicine, Department of Clinical and Chemical Pathology, Fayoum University, Fayoum, Egypt., Khalefa AA; Faculty of Medicine, Department of Physiology, Zagazig University, Zagazig, Egypt., Hussein RA; Faculty of Medicine, Department of Medical Microbiology and Immunology, Zagazig University, Zagazig, Egypt., Elkhateeb AF; Faculty of Medicine, Department of Critical Care, Fayoum University, Fayoum, Egypt., Mohamed EA; Faculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Fayoum University, Fayoum, Egypt.
Jazyk:	angličtina
Zdroj:	PloS one [PLoS One] 2024 Dec 27; Vol. 19 (12), pp. e0313042. Date of Electronic Publication: 2024 Dec 27 (Print Publication: 2024).
DOI:	10.1371/journal.pone.0313042
Abstrakt:	Background: The SARS-CoV-2 virus's frequent mutations have made disease control with vaccines and antiviral drugs difficult; as a result, there is a need for more effective coronavirus drugs. Therefore, detecting the expression of various diagnostic biomarkers, including ncRNA in SARS-CoV2, implies new therapeutic strategies for the disease. Aim: Our study aimed to measure NEAT-1, miR-374b-5p, and IL6 in the serum of COVID-19 patients, demonstrating the correlation between target genes to explore the possible relationship between them. Also, the association between target genes and patients' clinical findings and radiological severity indices will be explored. Patients and Methods: The current study included 48 COVID-19-infected individuals and 40 controls. Quantitative real-time PCR (qPCR) was performed to detect lncRNA NEAT-1 and miRNA374b-5p fold change (FC) in the participants' sera. Enzyme-Linked Immune Sorbent Assay (ELISA) is used to detect IL6. Results: Our results showed statistical significance with lower levels of (NEAT-1) [ median (range) = 0.08 (0.001-0.602)], and (miR374b-5p) [ median (range) = 0.14 (.01-7.16)] while higher IL-6 levels [ median (range) = 41.3 (7.2-654) pg/ml] when compared to controls with p-value <0.001. Serum level of NEAT-1 correlates negatively with IL-6 level (r = -.317, P = .008). ROC curve analysis revealed that sensitivity and specificity tests for NEAT-1 and IL-6 levels in the diagnosis of cases illustrated a sensitivity of (100% and 97.9%) and a specificity of (85% and 100%) at cut-off values (0.985 and 12.55), respectively. In comparison, miR374b-5p showed sensitivity and specificity of around 85% in distinguishing COVID-19 patients from controls. No significant association was detected between target genes and radiological severity indices. Conclusions: Our study is the first to detect decreased NEAT-1 and miR374b-5p expression in COVID-19 patients' serum. There was also an increase in IL6 levels. There is a negative correlation between NEAT-1 and IL6 in COVID-19 patients. Competing Interests: The authors have declared that no competing interests exist. (Copyright: © 2024 Ali et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze:	MEDLINE
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