Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation.

Autor: García JE; Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria (INTA), Nicolás Repetto y de los Reseros S/N, Hurlingham, B1713, Buenos Aires, Argentina., Pagnussat LA; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina.; Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC), Vieytes 3103, B7602, Mar del Plata, Buenos Aires, Argentina., Amenta MB; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina., Casanovas EM; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina., Diaz PR; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 2290 C1425, Godoy Cruz, CABA, Argentina., Labarthe MM; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 2290 C1425, Godoy Cruz, CABA, Argentina., Martino MV; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina., Groppa MD; Instituto de Química y Fisicoquímica Biológicas (IQUIFIB), CONICET-Universidad de Buenos Aires (UBA), C1113, Junin 956, Buenos Aires, Argentina., Creus CM; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina., Maroniche GA; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina. gmaroniche@mdp.edu.ar.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 2290 C1425, Godoy Cruz, CABA, Argentina. gmaroniche@mdp.edu.ar.
Jazyk: angličtina
Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2024 Dec 27; Vol. 108 (1), pp. 543. Date of Electronic Publication: 2024 Dec 27.
DOI: 10.1007/s00253-024-13391-0
Abstrakt: Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought. KEY POINTS: • Trehalose is required by Az19 for full tolerance to salt-, osmotic-, and UV-stress. • A restriction in trehalose accumulation alters Az19 normal cell physiology. • Trehalose contributes to Az19-induced maize growth promotion under drought.
Competing Interests: Declarations. Ethical approval: This article complies with ethical standards and does not contain any studies with human participants or animal performed by any of the authors. Conflict of interest: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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