Novel function of Hox13 in regulating outgrowth of the newt hindlimb bud through interaction with Fgf10 and Tbx4.

Autor: Tozawa S; Graduate School of Medical Sciences, Tottori University Yonago, Tottori, Japan., Matsubara H; Division of Developmental Biology, School of Life Sciences, Faculty of Medicine, Tottori University Yonago, Tottori, Japan., Minamitani F; Division of Developmental Biology, School of Life Sciences, Faculty of Medicine, Tottori University Yonago, Tottori, Japan., Kamei Y; Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan., Saida M; Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan., Asao M; Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan., Suzuki KT; Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan., Matsunami M; Graduate School of Medicine, University of the Ryukyus, Nishihara-Cho, Japan., Shigenobu S; Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan., Hayashi T; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.; Amphibian Research Center, Hiroshima University, Higashi-Hiroshima, Japan., Abe G; Graduate School of Medical Sciences, Tottori University Yonago, Tottori, Japan.; Division of Developmental Biology, School of Life Sciences, Faculty of Medicine, Tottori University Yonago, Tottori, Japan., Takeuchi T; Graduate School of Medical Sciences, Tottori University Yonago, Tottori, Japan.; Division of Developmental Biology, School of Life Sciences, Faculty of Medicine, Tottori University Yonago, Tottori, Japan.
Jazyk: angličtina
Zdroj: Development, growth & differentiation [Dev Growth Differ] 2024 Dec 26. Date of Electronic Publication: 2024 Dec 26.
DOI: 10.1111/dgd.12952
Abstrakt: 5'Hox genes regulate pattern formation along the axes of the limb. Previously, we showed that Hoxa13/Hoxd13 double-mutant newts lacked all digits of the forelimbs during development and regeneration, showing that newt Hox13 is necessary for digit formation in development and regeneration. In addition, we found another unique phenotype. Some of the Hox13 crispant newts showed hindlimb defects, in which whole or almost whole hindlimbs were lost, suggesting a novel function of Hox13 in limb development. Using germline mutants, we showed that mutation in Hox13 led to hindlimb defects. The limb buds of Hox13 crispants formed, however, did not show outgrowth. Expression of Fgf10 and Tbx4, which are involved in limb outgrowth, decreased in the hindlimb buds of Hox13 crispants. In addition, hindlimb defects were observed in both Fgf10 and Tbx4 crispant newts. Finally, Fgf10 and Tbx4 interacted with Hox13 genetically. Our results revealed a novel function of Hox13 in regulating the outgrowth of the newt hindlimb bud through interaction with Fgf10 and Tbx4.
(© 2024 The Author(s). Development, Growth & Differentiation published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Developmental Biologists.)
Databáze: MEDLINE
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