Extracellular Optogenetics to Interrogate Unmodified Primary Cell Functions.

Autor:	Jaeger M; Aix Marseille Université, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, CIML, Turing Center for Living Systems, Marseille, France., Vincentelli R; Aix Marseille Université, Centre National de la Recherche Scientifique, AFMB, Marseille, France., Lasserre R; Aix Marseille Université, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Institut Paoli-Calmettes, CRCM, Turing Center for Living Systems, Marseille, France. remi.lasserre@inserm.fr.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2840, pp. 163-174.
DOI:	10.1007/978-1-0716-4047-0_12
Abstrakt:	Optogenetics often requires genetic modification of the target cells to enable the expression of specific optogenetic tools, making it difficult to study primary cells in their native state. We have recently generated a fully extracellular optogenetic system for reversible light control of T cell receptor (TCR) activation on murine naïve T cells, a cell model that is very difficult to manipulate genetically. This molecular system is very versatile and can be easily modified to study different cell systems in different species. Here we describe how to produce and use this extracellular optogenetic system to manipulate primary T cell activation. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu