Na + -Complexed Dendritic Polyglycerols for Recovery of Frozen Cells and Their Network in Media.

Autor: Won TK; Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Republic of Korea., Shin A; Department of Chemistry, Yonsei University, Seoul, 03722, Republic of Korea., Lee SY; Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Republic of Korea., Kim BS; Department of Chemistry, Yonsei University, Seoul, 03722, Republic of Korea., Ahn DJ; Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Republic of Korea.; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2024 Dec 26, pp. e2416304. Date of Electronic Publication: 2024 Dec 26.
DOI: 10.1002/adma.202416304
Abstrakt: In this study, a novel phenomenon is identified where precise control of topology and generation of polyglycerol induce the retention of Na + ions in biological buffer systems, effectively inhibiting ice crystal growth during cryopreservation. Unlike linear and hyperbranched counterparts, densely-packed hydroxyl and ether groups in 4th-generation dendritic polyglycerol interact with the ions, activating the formation of hydrogen bonding at the ice interface. By inhibiting both intra- and extracellular ice growth and recrystallization, this biocompatible dendritic polyglycerol proves highly effective as a cryoprotectant; hence, achieving the cell recovery rates of ≈134-147%, relative to those of 10% dimethyl sulfoxide, which is a conventional cryoprotectant for human tongue squamous carcinoma (HSC-3) cell line and human umbilical vein endothelial (HUVEC) cells. Further, it successfully recovers the network-forming capabilities of HUVEC cells to ≈89% in tube formation after thawing. The Na + ion retention-driven ice-growth inhibition activity in biological media highlights the unique properties of dendritic polyglycerol and introduces a new topological concept for cell-cryoprotectant development.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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