Prenatal Methamphetamine Exposure Impairs Helping Behaviour in Male Offspring: The Possible Role of miR-223 and NLRP3 Inflammasomes in the Amygdala.

Autor: Khodagholi F; Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Dezfouli MA; Department of Neurology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran., Yazdanfar N; Department of Neurology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran., Rashidi SK; Department of Medical Biotechnology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran., Meymand AZ; Sina Truma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran., Javadpour P; Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Mirbehbahani SH; Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Zare N; Department of Anatomical Sciences and Cognitive Neurosciences, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Jazyk: angličtina
Zdroj: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience [Int J Dev Neurosci] 2025 Feb; Vol. 85 (1), pp. e10410.
DOI: 10.1002/jdn.10410
Abstrakt: The increasing prevalence of methamphetamine abuse among women, particularly pregnant females, is a global concern. Methamphetamine can readily cross anatomical barriers like the blood-placenta barrier and cause detrimental impacts on the growing fetus. The current research evaluated the effects of prenatal methamphetamine exposure on helping behaviour and neuroinflammatory cascade in the amygdala of male offspring. On the tenth day of pregnancy, female rats received either saline or methamphetamine (5 mg/kg) until delivery. Once the offspring reached 21 days of age, the male ones were sep arated from their mothers and housed with normal male rats. An empathy-like behaviour test, which measured helping behaviour towards the cage mate, was conducted. The expression levels of miR-223-3p, NLRP3, Caspase 1, and gasdermin D (GSDMD) were evaluated in the amygdala of male offspring. Moreover, interleukin-1β (IL-1β) protein level was measured. Findings of this study revealed that male offspring exposed to methamphetamine during pregnancy had impaired helping behaviour. At the molecular level, prenatal methamphetamine exposure decreased miR-223-3p and increased inflammasome signaling by raising the levels of NLRP3, caspase-1, and GSDMD along with IL-1β levels. These findings indicate that prenatal methamphetamine exposure impairs emotional behaviour and activates inflammasome pathway in the amygdala.
(© 2024 International Society for Developmental Neuroscience.)
Databáze: MEDLINE
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