Untargeted fast proteomics analysis using UPLC-Orbitrap HRMS for halal authentication of meat and meat products.

Autor: Suratno S; Research Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia. Electronic address: nanosan80@gmail.com., Windarsih A; Research Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia., Anggraeni AS; Research Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia., Alam LPM; Directorate of Laboratory Management, Research Facilities, and Science and Technology Park, National Research and Innovation Agency (BRIN), Playen Area, Gunungkidul, Yogyakarta 55861, Indonesia., Warmiko HD; Corpora Science, PT. Wiralab, Jln. Prof. Herman Yohanes No.988 Terban, Gondokusuman, Yogyakarta 55223, Indonesia., Jenie SNA; Research Center for Chemistry, National Research and Innovation Agency (BRIN), Serpong 15314, Indonesia., Novianty H; Research Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia., Ulumuddin YI; Research Center for Oceanography, National Research and Innovation Agency (BRIN), Jakarta 14430, Indonesia., Marmita A; Halal Product Assurance Organizing Agency (BPJPH), Jakarta 13560, Indonesia., Ayuni NPS; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Ganesha University of Education, Singaraja, Bali 81116, Indonesia., Indrianingsih AW; Research Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia., Rohman A; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2024 Dec 23, pp. 105369. Date of Electronic Publication: 2024 Dec 23.
DOI: 10.1016/j.jprot.2024.105369
Abstrakt: The authenticity of halal meat is a global issue because pork adulteration occurs. Certain religions, such as Islam and Judaism, prohibit the use of pork in food products. The purpose of this study was to evaluate the volume of trypsin with 10, 50 and 100 μL (20 μg/100 μL) and the digestion time from overnight to 30-120 min to establish a fast and straightforward procedure on proteomic analysis for halal authentication of meat and meat products. The method was applied to raw meat and processed pork products. The results show that 30 min digestion time and 50 μL of trypsin could detect specific peptides from pork. The proteins such as L-lactate dehydrogenase (D2SW96), haemoglobin subunit beta (F1RII7), carbonic anhydrase (A0A4XIUCS1), and myosin-1 (A0A481AX92) could be the alternative protein that contains specific peptide from pork that could be used as peptide biomarker in raw pork meat. Two peptides from haemoglobin subunit beta (F1RII7) protein heat stable peptide biomarkers were not previously reported. The method is proven for fast analysis, simple protein extraction, and rapid identification of specific pork peptides in raw meat and processed pork products. SIGNIFICANCE: The authenticity of halal meat is a global issue because pork adulteration occurs. Certain religions, such as Islam and Judaism, prohibit the use of pork in food products. Pork is frequently used as an adulterant in high-quality, high-priced meats such as beef, fish meat slices, lamb, or other meat to increase profits because pork is less expensive than the other meats. The purpose of this study was to evaluate the volume of trypsin and the digestion time to establish a fast and straightforward procedure on proteomic analysis for halal authentication of meat and meat products. The results show that 30 min digestion time and 50 μL of trypsin could detect specific peptides from pork. The proteins such as L-lactate dehydrogenase (D2SW96), haemoglobin subunit beta (F1RII7), carbonic anhydrase (A0A4XIUCS1), and myosin-1 (A0A481AX92) could be the alternative protein that contains specific peptide from pork that could be used as peptide biomarker in raw pork meat. To the best of our knowledge, this is the first report that studied on fast analysis, simple protein extraction, and rapid identification of specific pork peptides in raw meat and processed pork products.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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