The effect of silicon supplementation and drought stress on the deposition of callose and chemical components in the cell walls of the Brassica napus roots.

Autor: Diana SG; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, Kraków, 30-239, Poland. d.saja@ifr-pan.edu.pl., Kamila GJ; Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, Katowice, 40-032, Poland. kamila.godel@gmail.com., Ewa K; Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, Katowice, 40-032, Poland., Małgorzata KK; Department of Plant Experimental Biology and Biotechnology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, Gdańsk, 80-308, Poland., Monika T; Department of Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University, Gronostajowa 9, Kraków, 30-387, Poland., Emilia G; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, Kraków, 30-239, Poland., Kaja S; Department of Ecology, Institute of Botany, Jagiellonian University, Gronostajowa 3, Kraków, 30-387, Poland., Magdalena R; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, Kraków, 30-239, Poland., Karolina U; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, Kraków, 30-239, Poland., Monika K; Department of Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University, Gronostajowa 9, Kraków, 30-387, Poland., Marta LK; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, Kraków, 30-239, Poland.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2024 Dec 26; Vol. 24 (1), pp. 1249. Date of Electronic Publication: 2024 Dec 26.
DOI: 10.1186/s12870-024-05967-9
Abstrakt: Background: Silicon has an important role in regulating water management in plants. It is deposited in cell walls and creates a mechanical barrier against external factors. The aim of this study was to determine the role of silicon supplementation in the synthesis and distribution of callose in oilseed rape roots and to characterize the modifications of cell wall structure of these organs after exposure to drought stress. Histological and ultrastructural analyses were performed to determine the changes in the distribution of arabinogalactan proteins, pectins, and extensin in roots of Brassica napus growing under drought and supplemented with silicon. Callose deposition and the accumulation of callose synthase protein were assessed, followed by transcriptional analysis of callose synthase genes.
Results: The results showed that silicon supplementation under drought conditions alter the direction of cortex cell differentiation, promoting fiber formation and proliferation of callose-depositing cells in the roots of the tested plants. This was reflected in an increase in the level of callose synthase and a decrease in the transcriptional activity of the gene encoding this enzyme, indicating regulation based on negative feedback under drought stress. The changes in abundance and distribution of investigated arabinogalactan proteins, pectins and extensin in roots of Si supplemented plants growing under drought stress were observed, indicating cell walls remodeling.
Conclusion: Silicon supplementation in oilseed rape roots induced significant changes in cell wall composition, including increased callose deposition and altered pectins and arabinogalactan proteins distribution. These modifications, along with the formation of fibres in the root cortex, likely contribute to enhanced cell wall strength providing a physical barrier against water loss and mechanical stress, as a probable defence mechanism induced during drought stress.
Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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