Microbial diversity and community structure of microalgae (Nannochloropsis oculata) and rotifer (Brachionus plicatilis) in aquaculture by 16S rRNA amplicon-based sequencing.

Autor: Carranza-Quirós A; Universidad Nacional, Escuela de Ciencias Biológicas, Laboratorio de Análisis Genómico (LAGen), Heredia, 83-3000, Costa Rica., Solano-Campos F; Universidad Nacional, Escuela de Ciencias Biológicas, Laboratorio de Biotecnología de Plantas, Heredia, 83-3000, Costa Rica., Carvajal-Oses M; Universidad Nacional, Escuela de Ciencias Biológicas, Estación de Biología Marina 'Juan Bertoglia Richards', PuntarenasCentro, 60101, Costa Rica., Chacón-Guzmán J; Universidad Nacional, Escuela de Ciencias Biológicas, Estación de Biología Marina 'Juan Bertoglia Richards', PuntarenasCentro, 60101, Costa Rica.; Universidad Nacional, Escuela de Ciencias Biológicas, Programa Parque Marino del Pacífico, Puntarenas, Centro, 60101, Costa Rica., Navarrete P; Microbiology and Probiotic Laboratory, Institute of Nutrition and Food Technology (INTA), University of Chile, Avenida el Líbano 5524, 7830490, Macul, Santiago, Chile., Reinoso S; Centro Nacional de Acuicultura e Investigaciones Marinas, CENAIM, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, 090211, Guayaquil, Ecuador., Umaña-Castro R; Universidad Nacional, Escuela de Ciencias Biológicas, Laboratorio de Análisis Genómico (LAGen), Heredia, 83-3000, Costa Rica. rodolfo.umana.castro@una.ac.cr.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2024 Dec 24; Vol. 118 (2), pp. 44. Date of Electronic Publication: 2024 Dec 24.
DOI: 10.1007/s10482-024-02053-w
Abstrakt: Live cultures, including Nannochloropsis oculata and Brachionus plicatilis, are essential in aquaculture due to its economic and nutritional value for commercial fish species. Pathogens and probiotics can be introduced to aquaculture systems by live feed, with variations in abundance influenced by environmental physicochemical parameters. To investigate this, amplicon sequencing of the V3-V4 region of the 16S rRNA was conducted using Illumina MiSeq to elucidate bacterial abundances and their variations in response to changes in physicochemical parameters in live feed cultures. Results revealed that pathogens like, Tenacibaculum, predominantly found in rotifer cultures, was inhibited under dissolved oxygen levels of 6.87-8.19 mg/L and pH levels of 8.63-9.23. Winogradskyella, mainly present on day 1 of the microalgae culture, thrived under these same conditions. Potential probiotics were also analyzed. Phaeodactylibacter, primarily found on day 1 of microalgae cultures, was favored by dissolved oxygen levels of 7.11-7.68 mg/L and pH levels of 8.63-9.23 but was inhibited by nitrite levels of 0.46-0.5 mg/L. Overall, dissolved oxygen was the key parameter modulating microbial diversity. This study shows that optimizing environmental parameters can enhance culture health by promoting probiotics and reducing harmful bacteria.
Competing Interests: Declarations. Conflict of interest: The authors declare that they have no known financial or personal competing interests. Ethics approval: Not applicable. Consent for publication: Not applicable.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
Externí odkaz: