TAG-1 Regulates NRP1 in Schwann Cells and Participates in Regulating Nerve Regeneration in Rats with Sciatic Nerve Crush Injury.

Autor: Liu PS; Department of Spinal Surgery, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China.; Yantai Key Laboratory of Repair and Reconstruction of Bone & Joint, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China., An X; Department of Emergency, Yantai Yuhuangding Hospital, Yantai, 264000, China., Zhou QS; Department of Spinal Surgery, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China.; Yantai Key Laboratory of Repair and Reconstruction of Bone & Joint, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China., Sun XR; Department of Spinal Surgery, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China.; Yantai Key Laboratory of Repair and Reconstruction of Bone & Joint, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China., Wang HM; Department of Spinal Surgery, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China.; Yantai Key Laboratory of Repair and Reconstruction of Bone & Joint, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China., Xu XD; Department of Spinal Surgery, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China.; Yantai Key Laboratory of Repair and Reconstruction of Bone & Joint, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, 264003, China., Li M; Department of Spinal Surgery, Yantai Hospital of Traditional Chinese Medicine, No.39, Xingfu Road, Zhifu District, Yantai, 264000, China. Jgjzwzsy@163.com.
Jazyk: angličtina
Zdroj: Neurochemical research [Neurochem Res] 2024 Dec 24; Vol. 50 (1), pp. 65. Date of Electronic Publication: 2024 Dec 24.
DOI: 10.1007/s11064-024-04310-w
Abstrakt: Schwann cells (SCs) are necessary for peripheral nerve regeneration due to their plasticity and trophic supply after sciatic nerve injury (SNI). However, the multiple adaptations of SCs are still poorly understood. This study explored the effects of transient axonal glycoprotein type-1 (TAG-1) on cell migration and neuropilin1 (NRP1) expression in SCs and examined the impact of TAG-1 on nerve regeneration in rats with SNI. The expression of TAG-1 and NRP1 in SCs, RSC96 cells, was measured using co-immunoprecipitation and immunofluorescence. TAG-1 silence in RSC96 cells was established by TAG-1 siRNA transfection. The effects of TAG-1 silence on cell migration and NRP1 expression were measured in cells. Male adult Wistar rats suffered sciatic nerve crush injury and were treated with exogenous TAG-1 protein. The sciatic function was observed every week. The histological changes of sciatic nerves and expressions of S100β, NRP1, GAP43, and NCAM in the nerves were observed after injury 28 days. The TAG-1 and NRP1 were expressed in the RSC96 cells, and there was an interaction between TAG-1 and NRP1. TAG-1 silence suppressed the cell migration and NRP1 expression in cells. In rats with SNI, the TAG-1 treatment improved the sciatic function and promoted nerve regeneration by increasing the expressions of S100β, NRP1, GAP-43, and NCAM in the nerves. This study showed that TAG-1 regulated cell migration and NRP1 expression in SCs, and TAG-1 treatment might be a strategy for nerve regeneration after the SNI.
Competing Interests: Declarations. Conflict of interest: The authors have no conflicts of interest to declare. Ethical Approval: All experiment processes were agreed upon with the Ethics Committee of Yantai Yuhuangding Hospital (Approval No. 2024 − 472). All experimental procedures were performed following the ARRIVE guidelines for the animal experiments.
(© 2024. The Author(s).)
Databáze: MEDLINE
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