Machine learning-enhanced assessment of potential probiotics from healthy calves for the treatment of neonatal calf diarrhea.

Autor:	Zhai Y; Department of Animal Sciences, University of Florida, Gainesville, FL, United States.; Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States., Kim M; Department of Animal Sciences, University of Florida, Gainesville, FL, United States.; Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States.; Department of Food Science and Biotechnology, Kyung Hee University, Seoul, Republic of Korea., Fan P; Department of Animal Sciences, University of Florida, Gainesville, FL, United States., Rajeev S; Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States., Kim SA; Department of Food Science and Biotechnology, Ewha Womans University, Seoul, Republic of Korea., Driver JD; Department of Animal Sciences, University of Florida, Gainesville, FL, United States., Galvão KN; Department of Large Animal Clinical Sciences, University of Florida, Gainesville, FL, United States., Boucher C; Department of Computer and Information Science and Engineering, University of Florida, Gainesville, FL, United States., Jeong KC; Department of Animal Sciences, University of Florida, Gainesville, FL, United States.; Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Dec 09; Vol. 15, pp. 1507537. Date of Electronic Publication: 2024 Dec 09 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1507537
Abstrakt:	Neonatal calf diarrhea (NCD) remains a significant contributor to calf mortality within the first 3 weeks of life, prompting widespread antibiotic use with associated concerns about antimicrobial resistance and disruption of the calf gut microbiota. Recent research exploring NCD treatments targeting gut microbiota dysbiosis has highlighted probiotic supplementation as a promising and safe strategy for gut homeostasis. However, varying treatment outcomes across studies suggest the need for efficient treatment options. In this study, we evaluated the potential of probiotics Limosilactobacillus reuteri , formally known as Lactobacillus reuteri , isolated from healthy neonatal calves to treat NCD. Through in silico whole genome analysis and in vitro assays, we identified nine L. reuteri strains, which were then administered to calves with NCD. Calves treated with L. reuteri strains shed healthy feces and demonstrated restored gut microbiota and normal animal behavior. Leveraging a machine learning model, we evaluated microbiota profiles and identified bacterial taxa associated with calf gut health that were elevated by L. reuteri administration. These findings represent a crucial advancement towards sustainable antibiotic alternatives for managing NCD, contributing significantly to global efforts in mitigating antimicrobial resistance and promoting overall animal health and welfare. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Zhai, Kim, Fan, Rajeev, Kim, Driver, Galvão, Boucher and Jeong.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu