Dual suppression of Glossina pallidipes using entomopathogenic fungal-based biopesticides and sterile insect technique.

Autor: Ombura FLO; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya., Abd-Alla AMM; Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, Austria., Akutse KS; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; Unit of Environmental Sciences and Management, North-West University, Potchefstroom, South Africa., Runo S; Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya., Mireji PO; Biotechnology Research Institute, Kenya Agricultural and Livestock Research Organization, Kikuyu, Kenya.; Centre for Geographic Medicine Research Coast, Kenya Medical Research Institute, Kilifi, Kenya., Bateta R; Biotechnology Research Institute, Kenya Agricultural and Livestock Research Organization, Kikuyu, Kenya., Otiwi JE; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya., Ajene IJ; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya., Khamis FM; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; Department of Zoology and Entomology, University of Pretoria, Pretoria, South Africa.
Jazyk: angličtina
Zdroj: Frontiers in microbiology [Front Microbiol] 2024 Dec 09; Vol. 15, pp. 1472324. Date of Electronic Publication: 2024 Dec 09 (Print Publication: 2024).
DOI: 10.3389/fmicb.2024.1472324
Abstrakt: Tsetse flies and trypanosomosis significantly impact bovine production and human health in sub-Saharan Africa, exacerbating underdevelopment, malnutrition, and poverty. Despite various control strategies, long-term success has been limited. This study evaluates the combined use of entomopathogenic fungi (EPF) and the sterile insect technique (SIT) to combat tsetse flies. Eleven EPF isolates were tested against teneral males of Glossina pallidipes , focusing on mortality rates, radial growth, and impacts on fly fitness. Temperature effects on conidial growth, sporulation, and spore yield of SIT-compatible/tolerant strains were also assessed. The fungal isolates significantly influenced mortality rates in both unirradiated and irradiated (SIT-treated) males ( p  < 0.0001). Metarhizium anisopliae strains ICIPE 20, ICIPE 32, ICIPE 41, ICIPE 62, ICIPE 78, and Beauveria bassiana ICIPE 603 showed higher SIT compatibility/tolerance with LT 50 values of 11-30 days, compared to other more virulent isolates with LT 50 values of 4-9 days. Temperature significantly affected the radial growth of SIT-compatible EPF strains ( p  < 0.0001), with M. anisopliae ICIPE 78 exhibiting the fastest conidia growth at 25°C. Spore yield varied significantly across temperatures (15-40°C), and the thermal range for conidia germination of SIT-compatible strains was 8.1-45.4°C, with an optimal range of 26.7-31.1°C. Moreover, infected unirradiated females and irradiated males (donors) successfully transmitted conidia to untreated flies (receivers) without significant differences in survival rates ( p  = 0.6438) and no observed sex dimorphism. Our findings highlight the potential of combining EPF and SIT as a novel dual approach that could effectively and synergistically suppress tsetse fly populations.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Ombura, Abd-Alla, Akutse, Runo, Mireji, Bateta, Otiwi, Ajene and Khamis.)
Databáze: MEDLINE
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