The Effects of Acidified Nitrite on Wound Healing in Streptozotocin-Induced Diabetic Mice.
| Autor: | Ghorbani M; Tissue Engineering and Regenerative Medicine Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran., Ghajari G; Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran., Jalali Kondori B; Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.; Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, Iran. |
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| Jazyk: | angličtina |
| Zdroj: | Advanced biomedical research [Adv Biomed Res] 2024 Oct 28; Vol. 13, pp. 97. Date of Electronic Publication: 2024 Oct 28 (Print Publication: 2024). |
| DOI: | 10.4103/abr.abr_115_24 |
| Abstrakt: | Background: Diabetes mellitus (DM) is one of the most common metabolic diseases in the world. Studies have shown that nitric oxide (NO) promotes re-epithelialization and stimulates angiogenesis and neovascularization. This study aimed to investigate the effect of exogenous NO on diabetic wound healing. Materials and Methods: This study was performed on 63 male BALB/c mice. For type 2 diabetes induction, the animals were fed a high-fat diet followed by a single dose of streptozotocin (STZ) (35 mg/kg) injection intraperitoneally. Acidified nitrite cream was prepared with 3.0% (w/v) sodium nitrite (SN) and 4.5% (w/v) citric acid monohydrate, respectively, in the aqueous cream base. Histopathological examinations were performed using hematoxylin and eosin and Masson's trichrome staining. Results: The results showed that in the silver sulfadiazine-treated group, the size of the wound surface on the 7 th day was significantly ( P < 0.05) reduced compared to the control group. There was a significant ( P < 0.005) decrease in the size of the wound in the SN-treated group on days 7 and 14 compared to the control group. The results of histopathological studies also showed that re-epithelialization and granulation in the diabetic wound site increased in the groups treated with acidified nitrite cream compared to other groups. Conclusion: The use of topical acidified nitrite cream increases the speed of wound healing and it accelerates the healing of diabetic wounds in mice by causing a delay in the inflammation process and increasing the speed of re-epithelialization. Competing Interests: There are no conflicts of interest. (Copyright: © 2024 Advanced Biomedical Research.) |
| Databáze: | MEDLINE |
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