| Autor: |
Thornell IM; Department of Internal Medicine and Pappajohn Biomedical Institute Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA., Lei L; Stead Family Department of Pediatrics and Pappajohn Biomedical Institute Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA., McCray PB Jr; Stead Family Department of Pediatrics and Pappajohn Biomedical Institute Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.; Department of Microbiology and Immunology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA., Welsh MJ; Department of Internal Medicine and Pappajohn Biomedical Institute Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA.; Department of Molecular Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA.; Howard Hughes Medical Institute, University of Iowa, Iowa City, USA. |
| Abstrakt: |
Pulmonary ionocytes express high levels of cystic fibrosis transmembrane conductance regulator (CFTR) channels. When studied using the short-circuit current technique, ionocytes produce CFTR-dependent short-circuit currents consistent with Cl - secretion. However, when studied without a voltage-clamp, data indicate that ionocytes absorb Cl - . In this review, we resolve these seemingly conflicting findings by considering the different transepithelial voltages and resultant movement of Cl - during short-circuit and physiological open-circuit conditions. This analysis indicates that behavior under short-circuit conditions cannot be directly extrapolated to infer behavior under physiologic conditions. Finally, we discuss the potential role for basolateral Cl - channels in controlling absorption and secretion in ionocytes. |
