Association Between Stress-Induced Weight Loss and Autophagy-Related Gene Expression in the Hippocampus and Midbrain of Depression Model Mice.

Autor: Mori H; Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Toon, Japan., Yoshino Y; Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Toon, Japan., Okano M; Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Toon, Japan., Funahashi Y; Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Toon, Japan., Kumon H; Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Toon, Japan., Ochi S; Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Toon, Japan., Iga JI; Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Toon, Japan., Ueno SI; Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Toon, Japan.
Jazyk: angličtina
Zdroj: Neuropsychopharmacology reports [Neuropsychopharmacol Rep] 2025 Mar; Vol. 45 (1), pp. e12515.
DOI: 10.1002/npr2.12515
Abstrakt: Aim: Recent studies have implicated autophagy in both weight regulation and depression. This study aimed to investigate the relationship between stress-induced weight loss and autophagy-related gene expression in a mouse model of depression.
Method: Male C57BL/6 mice were subjected to a chronic immobilization stress (CIS) protocol for 14 days to induce depressive-like behavior. Body weight was measured before and after the CIS, and depressive-like behavior was assessed using the tail suspension test (TST). The expression levels of autophagy-related genes (Atg5, Atg7, Atg12, Becn1, Mmp9, Fkbp5, and Map1lc3b) in the hippocampus and midbrain were evaluated using reverse transcription-quantitative PCR (RT-qPCR). Serum cortisol levels were also measured.
Results: The CIS resulted in significant weight loss and increased immobility time in the TST, indicating depressive-like behavior. Serum cortisol levels were not different between CIS-depression model and control mice. In the hippocampus, the expression levels of Fkbp5, Mmp9, and Map1lc3b were significantly higher in CIS-depression model mice than in control mice. In the midbrain, the expression levels of Fkbp5 and Mmp9 were significantly higher in CIS-depression model mice than in control mice. Increased autophagy-related gene expressions in CIS-depression model mice were consistent with the previous studies in the postmortem brains of patients with depression. A significant negative correlation was also found between Fkbp5 mRNA expression in the hippocampus and the weight change ratio before and after the CIS.
Conclusion: The findings suggest that enhanced autophagy may be related to the pathology of depression and that Fkbp5, an autophagy regulator, mediates stress-induced weight loss.
(© 2024 The Author(s). Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology.)
Databáze: MEDLINE
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