Posttraumatic hyperalgesia and associated peripheral sensitization after temporomandibular joint injury in mice.

Autor:	Alshanqiti I; Program in Dental Biomedical Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States.; Department of Neural and Pain Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States.; Department of Basic and Clinical Sciences, School of Dentistry, Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia., Son H; Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States.; Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, and Radiological Sciences, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States., Shannonhouse J; Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States., Hu J; Department of Neural and Pain Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States., Kumari S; Department of Neural and Pain Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States., Parastooei G; Department of Neural and Pain Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States., Raman S; Department of Neural and Pain Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States., Wang S; Department of Neural and Pain Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States., Ro JY; Department of Neural and Pain Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States.; Center to Advance Chronic Pain Research, University of Maryland at Baltimore, Baltimore, MD, United States.; Program in Neuroscience, University of Maryland at Baltimore, Baltimore, MD, United States., Kim YS; Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States.; Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, and Radiological Sciences, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States., Chung MK; Program in Dental Biomedical Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States.; Department of Neural and Pain Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States.; Center to Advance Chronic Pain Research, University of Maryland at Baltimore, Baltimore, MD, United States.; Program in Neuroscience, University of Maryland at Baltimore, Baltimore, MD, United States.
Jazyk:	angličtina
Zdroj:	Pain [Pain] 2024 Dec 17. Date of Electronic Publication: 2024 Dec 17.
DOI:	10.1097/j.pain.0000000000003498
Abstrakt:	Abstract: Temporomandibular disorder (TMD) is the most prevalent painful condition in the craniofacial area. Recent studies have suggested that external or intrinsic trauma to the temporomandibular joint (TMJ) is associated with the onset of painful TMD in patients. Here, we investigated the effects of TMJ trauma through forced-mouth opening (FMO) in mice to determine pain behaviors and peripheral sensitization of trigeminal nociceptors in both sexes. Forced-mouth opening increased mechanical pain as assessed by the von Frey test, with spontaneous pain-like behaviors assessed using the mouse grimace scale and anxiety-like behaviors assessed using the open-field test. Changes in pain-like behaviors were not different between male and female mice. However, in vivo GCaMP Ca2+ imaging of intact trigeminal ganglia (TG) showed modality- and sex-dependent changes. Forced-mouth opening increased spontaneous Ca2+ responses and mechanical hypersensitivity of TG neurons compared to the sham group, which was more pronounced in male mice. Forced-mouth opening also increased Ca2+ responses evoked by cold, heat, and capsaicin stimuli, which was not different between the sexes. In retrogradely labeled trigeminal TMJ afferents, FMO induced an increase in small-sized neuronal proportions with increased colocalization with calcitonin gene-related peptides and transient receptor potential vanilloid subtype 1, which was modestly sex dependent. These results suggest that TMJ injury leads to persistent posttraumatic hyperalgesia associated with peripheral sensitization of trigeminal nociceptors with distinct sex dependency. (Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.)
Databáze:	MEDLINE
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