Design and Characterization of Novel Naphthalimide Fluorescent Probe for H 2 S Detection in Human Serum.

Autor: Dandić A; Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, Osijek, 31000, Croatia., Samardžić M; Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, Osijek, 31000, Croatia., Budetić M; Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, Osijek, 31000, Croatia., Panić ID; Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, Osijek, 31000, Croatia., Drenjančević I; Department of Physiology and Immunology, Faculty of Medicine Osijek,, J. J. Strossmayer University of Osijek, J. Huttlera 4, Osijek, 31000, Croatia., Kolobarić N; Department of Physiology and Immunology, Faculty of Medicine Osijek,, J. J. Strossmayer University of Osijek, J. Huttlera 4, Osijek, 31000, Croatia., Mikle G; Department of General and Inorganic Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6, Pécs, 7624, Hungary.; Green Chemistry Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs, 7624, Hungary.; Research Group for Selective Chemical Syntheses, HUN-REN-PTE, Ifjúság útja 6., H-7624, Pécs, Hungary., Kovács B; Green Chemistry Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs, 7624, Hungary.; Institute of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, University of Pécs, Rokus utca 4, Pécs, 7624, Hungary., Széchenyi A; Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, Osijek, 31000, Croatia. szealex@kemija.unios.hr.; Green Chemistry Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs, 7624, Hungary. szealex@kemija.unios.hr.; Institute of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, University of Pécs, Rokus utca 4, Pécs, 7624, Hungary. szealex@kemija.unios.hr.
Jazyk: angličtina
Zdroj: Journal of fluorescence [J Fluoresc] 2024 Dec 23. Date of Electronic Publication: 2024 Dec 23.
DOI: 10.1007/s10895-024-04071-3
Abstrakt: In this work, a novel fluorescent probe (compound 2) based on the Intramolecular charge transfer (ICT) mechanism was designed and successfully applied to determine H 2 S in human serum. Fluorophore 1,8-naphthalimide was chosen, while the azide group was the recognition group for H 2 S determination. By introducing p-toluidine moiety on the imide part of the molecule, a donor-acceptor (D-A) conjugated system was formed. Prepared compound 2 was characterized using 1 H, 13 C NMR spectroscopy, and elemental analysis. Fluorescence spectra measurements were carried out, and several influences on fluorescence intensity were investigated, including pH, time dependence, selective response, and influence of H 2 S concentration. Conducted experiments, including the calculated detection limit of the prepared fluorescent probe, which was found to be 0.085 µmol·L - 1 , showing that compound 2 could be applied for H 2 S detection in human serum and could detect low micromolar concentrations of H 2 S. Finally, compound 2 was successfully applied to detect H 2 S in a human serum sample, whereby the concentration of H 2 S was 17.2 µmol·L - 1 . The accuracy of the H 2 S determination was confirmed with the standard addition method.
Competing Interests: Declarations. Competing Interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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