| Autor: |
Rodgers ML, Subramanian S, Fuess LE, He W, Scarpino SV, Roth-Monzón AJ, Jeffries D, Seignon M, Milligan-McClellan K, Carrier R, Steinel NC, Bolnick DI |
| Jazyk: |
angličtina |
| Zdroj: |
BioRxiv : the preprint server for biology [bioRxiv] 2024 Dec 09. Date of Electronic Publication: 2024 Dec 09. |
| DOI: |
10.1101/2024.12.06.627160 |
| Abstrakt: |
Rates of evolutionary change vary by gene. While some broad gene categories are highly conserved with little divergence over time, others undergo continuous selection pressure and are highly divergent. Here, we combine single-cell RNA sequencing (scRNAseq) with evolutionary genomics to understand whether certain cell types exhibit faster evolutionary divergence (using their characteristic genes), than other types of cells. Merging scRNAseq with population genomic data, we show that cell types differ in the rate at which their characteristic genes evolve, as measured by allele frequency divergence among many populations (F ST ) and between species (dN/dS ratios). Neutrophils, B cells, and fibroblasts exhibit elevated F ST at characteristic genes, while eosinophils in the intestine and thrombocytes in the head kidney exhibit lower F ST than the average for 1000 random genes. Gene network centrality also differed between immune- and non-immune-associated genes, and closeness centrality was positively related to gene F ST . These results highlight the value of merging single cell RNA sequencing technology with evolutionary population genomic data, and reveal that genes which define immune cell types exhibit especially rapid evolution. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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