Identification and Characterization of the Y-Organ of Orange Mud Crab Scylla Olivacea.

Autor: Achdiat M; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia., Fujaya Y; Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia., Fazhan H; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.; Center for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas, Penang, Malaysia.; Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity and Conservation, College of Marine Sciences, Beibu Gulf University, Guangxi, China., Rozaimi R; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia., Chung JS; Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, Maryland, USA., Wang Y; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China., Tan K; Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity and Conservation, College of Marine Sciences, Beibu Gulf University, Guangxi, China., Shu-Chien AC; Center for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas, Penang, Malaysia.; School of Biological Sciences, Universiti Sains Malaysia, Bayan Lepas, Penang, Malaysia.; Northland Aquaculture Centre, National Institute of Water and Atmospheric Research, Ruakaka, New Zealand., Maulidiani M; Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia., Waiho K; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.; Center for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas, Penang, Malaysia.; Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity and Conservation, College of Marine Sciences, Beibu Gulf University, Guangxi, China.
Jazyk: angličtina
Zdroj: Microscopy research and technique [Microsc Res Tech] 2024 Dec 22. Date of Electronic Publication: 2024 Dec 22.
DOI: 10.1002/jemt.24777
Abstrakt: The Y-organs (YOs) synthesize and secrete molting hormones, and thus regulate crustacean growth and reproduction. However, the YOs of the orange mud crab Scylla olivacea is yet to be described due to its minute size and ambiguous feature. This study describes the location, morphological characteristics, histology, and the changes of YOs at different molt stages, and examines in vitro ecdysteroid secreted by the YOs of S. olivacea. Mature male crabs (> 90 mm carapace width, CW) were used to identify the location of the YOs while male juveniles (50-65 mm CW) at three molt stages (intermoult, premoult, postmoult) were dissected for the characterization of 20-Hydroxyecdysone (20E) levels. The yellowish-white, compact, and oval-shaped YOs are located in pairs at the anterior cephalothorax region, beneath the mandibular external adductor muscle, anterior to the branchial chamber space, and around the end of the nerve tract. The YOs index and YO's cell diameter increased from postmoult to premoult; YO's diameter increased only during premoult stage. Under electron microscopy, the YO's surface morphology is rough, bumpy, network-like, and porous, with several lacunar system tubules. High concentrations of haemolymph 20E during premoult correlated with the changes in the characteristics of YOs at this stage. The presence of ecdysteroid in YOs was confirmed by high performance liquid chromatography (HPLC). The characteristics of the YOs of S. olivacea are similar to the YO descriptions of other brachyurans. The successful identification and characterization of YOs will spearhead future research on understanding its role in molt regulation of mud crabs, consequently contributing to the development and progress of crab culture and soft-shell crab industries.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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