Dual-mode exosome detection leveraging a nanozyme-active artificial receptor: PDA@Fe@Zn-based nucleic acid aptamer sensor.

Autor: Zhao L; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China., Kuang J; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China., Xiang K; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China., Gan J; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China., Zeng Y; Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, PR China., Zhang X; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China., Yan Y; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China., Zhang M; Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, PR China. Electronic address: zhangm@ecust.edu.cn., Zhang H; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China., Hu P; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China. Electronic address: huping@ecust.edu.cn.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Dec 21; Vol. 285, pp. 127380. Date of Electronic Publication: 2024 Dec 21.
DOI: 10.1016/j.talanta.2024.127380
Abstrakt: Exosomes, extracellular vesicles crucial for intercellular communication, are emerging as significant biomarkers for disease diagnosis, especially in cancer. This study presented a dual-mode exosome detection platform using polydopamine microspheres doped with iron and zinc ions (PDA@Fe@Zn). These materials served as both artificial receptors for nucleic acid aptamers and nanozymes with peroxidase-like activity. By integrating colorimetric and fluorescence detection, the platform enables cross-validation of results. PDA@Fe@Zn nanozymes catalyzed the TMB-H 2 O 2 reaction under acidic conditions, producing a colorimetric signal proportional to exosome concentration. Concurrently, the fluorescence of FAM-labeled aptamers was dynamically quenched by PDA@Fe@Zn and the presence of exosomes restores the fluorescence signal for a "turn-on" detection mode. DNase I amplified detection signals by cleaving bound exosomes for multiple cycles, achieving a limit of detection (LOD) of 4.7 × 10 4 particles/mL for colorimetric detection and 2.2 × 10 4 particles/mL for fluorescence detection. Notably, the colorimetric platform revealed that the relative expression of the CD63 protein on exosomes from breast cancer cells MCF-7 and MDA-MB-231 was approximately 2.7 and 2.4 times higher, respectively, than in normal breast cells MCF-10A; similar fold changes of 2.9 and 2.4 were observed with fluorescence detection, underscoring the robustness of the dual-mode system. The platform demonstrated rapid detection (within 30 min), high sensitivity, strong anti-interference capability, and the ability to distinguish exosomes from cancerous and normal cells effectively.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interestsPing Hu reports financial support was provided by National Natural Science Foundation of China. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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