Alcohol-based antisepsis without the use of chlorhexidine for arthroscopy in horses.

Autor: Nakamae Y; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave, Charlottetown Prince Edward Island, Canada C1A 4P3., Elce YA; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave, Charlottetown Prince Edward Island, Canada C1A 4P3., Saab ME; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave, Charlottetown Prince Edward Island, Canada C1A 4P3., McClure JT; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave, Charlottetown Prince Edward Island, Canada C1A 4P3., Doyle AJ; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave, Charlottetown Prince Edward Island, Canada C1A 4P3. Electronic address: ajdoyle@upei.ca.
Jazyk: angličtina
Zdroj: Journal of equine veterinary science [J Equine Vet Sci] 2024 Dec 19, pp. 105257. Date of Electronic Publication: 2024 Dec 19.
DOI: 10.1016/j.jevs.2024.105257
Abstrakt: Alcohol-based antisepsis has shown experimentally to be as effective as 4% chlorhexidine gluconate (CHG) at reducing bacterial counts (colony forming units; CFU) on equine skin. Our objectives were to determine the immediate and post-surgical reduction in CFU/mL on equine skin prepared with CHG-based or 70% isopropyl alcohol (IPA)-based (without CHG) protocols in a clinical setting with arthroscopic surgery. Our hypotheses were that the log 10 CFU/mL reduction would not significantly differ between protocols immediately after preparation or at the end of surgery. Six horses underwent a 40 min bilateral tarsocrural joint arthroscopy with each limb randomly assigned to Group A or B. Group A tarsocrural joints underwent a rough scrub using 4% CHG and a 5 min sterile scrub using 2% CHG. Group B underwent a rough scrub with neutral soap followed by a 90s sterile scrub with IPA. Samples were collected before rough scrub (T0), immediately after sterile scrub (T1), and end of surgery (T2). CFU/mL were determined in duplicate and were log-transformed and averaged. ANOVA models compared the immediate reduction (T0-T1) and sustained reduction (T0-T2) between treatment groups. The immediate and sustained log10CFU/mL reduction between groups was not different (P=0.46, P=0.42). Both groups achieved at least a 2-log immediate and sustained reduction. Limitations include small population size, short surgical duration, length of follow-up, and researchers were not blinded to treatment during sampling. This study demonstrates efficacy of IPA-based antisepsis, without the need for CHG, and supports further investigation in clinical surgery as an acceptable method of surgical site preparation.
Competing Interests: Declaration of competing interest None of the authors has any financial or personal relationship that could inappropriately influence or bias the content of the paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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