Discriminatory dose of nitroxynil and albendazole sulfoxide using a modified egg hatch test of Fasciola hepatica.

Autor: Pontarolo DV; Laboratory of Veterinary Clinical Parasitology. Federal University of Paraná, UFPR. Rua dos Funcionários, 1540, Curitiba, PR, CEP 80.035-050, Brazil. Electronic address: desi.medvet@gmail.com., Molento MB; Laboratory of Veterinary Clinical Parasitology. Federal University of Paraná, UFPR. Rua dos Funcionários, 1540, Curitiba, PR, CEP 80.035-050, Brazil. Electronic address: molento@ufpr.br.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2024 Dec 19; Vol. 268, pp. 108884. Date of Electronic Publication: 2024 Dec 19.
DOI: 10.1016/j.exppara.2024.108884
Abstrakt: Fasciolosis is a food-borne anthropozoonotic disease caused by Fasciola spp. that affects multiple hosts, including ruminants and humans. In vitro testing of anthelmintics is of interest to establish the drug's activity without the need for time-consuming and expensive in vivo assays. This study was set to establish a discriminatory dose (DD) by running a dose-titration in vitro experiment (egg hatch test, EHT) of albendazole sulfoxide (ABZ.SO) and nitroxynil (NTX) on eggs of a field strain of Fasciola hepatica. Eggs were recovered from adult parasites isolated from intact bovine livers obtained from a single farm in Paraná, Brazil (FhPar2022 strain) with no ABZ or NTX treatment history. Two hundred eggs were exposed to 18 and 14 concentrations of ABZ.SO and NTX, respectively, for 12h and incubated for 16 days. Egg development and integrity were determined every other day, establishing an index of morphological modification of the different phases. A concentration-dependent effect was observed for egg development in both compounds. ABZ.SO solutions prevent egg hatch, except for the two lowest concentrations. We observed no egg hatch at 6.250-100.0 μmol L -1 for NTX. NTX had an inhibition concentration of 50% (IC 50 ) of 0.043 μmol L -1 with a correlation coefficient of (R 2 ) 0.961. ABZ.SO had an IC 50 of 0.00099 μmol L -1 with a low R 2 of 0.417. Morphological damage was also associated with the increasing concentration of both drugs. Moreover, it was noted that most eggs that reached the eye spot type could hatch, except at 0.39 and 3.12 μmol L -1 of NTX. In ABZ.SO, hatching occurred only at 0.00038, 0.0007, and 0.0015 μmol L-1 concentrations. The obtained DDs of 0.043 μmol L -1 for NTX and 0.00099 μmol L -1 for ABZ.SO can be used to monitor efficacy in field isolates.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Desiree Vera Pontarolo reports financial support was provided by Coordination of Higher Education Personnel Improvement. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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