Isolation and characterization of Iwanai Valley virus, a new tick-borne nairovirus from Ixodes ovatus ticks in Hokkaido, Japan.

Autor: Matsumura R; Graduate School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa, 214-8571, Japan.; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan., Kobayashi D; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.; Management Department of Biosafety, Laboratory Animal, and Pathogen Bank, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan., Yamauchi T; Laboratory of Entomology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan., Park E; Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan., Nishino A; Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.; Joint Graduate School of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, Yamaguchi, 753-8511, Japan., Maeda K; Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan., Kasai S; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan., Itoyama K; Graduate School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa, 214-8571, Japan., Isawa H; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. hisawa@niid.go.jp.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2024 Dec 21; Vol. 170 (1), pp. 23. Date of Electronic Publication: 2024 Dec 21.
DOI: 10.1007/s00705-024-06195-0
Abstrakt: Most members of the genus Orthonairovirus, represented by Crimean-Congo hemorrhagic fever virus and Nairobi sheep disease virus, are tick-borne, and some have become a public health concern in recent years. Here, we report the isolation and genetic and biological characterization of a new orthonairovirus, designated as "Iwanai Valley virus" (IWVV), from Ixodes ovatus ticks in Hokkaido, Japan. The amino acid sequence of the viral nucleoprotein (NP) was found to be 34-45% identical to those of known orthonairoviruses. Phylogenetic analysis based on amino acid sequences of the viral NP revealed that IWVV is closely related to human-pathogenic tick-borne orthonairoviruses, including Songling virus, Tacheng tick virus 1, and Yezo virus, which were recently discovered in East Asia. BHK-21 (Syrian hamster kidney), Vero (African green monkey kidney), SW-13 (human adrenal adenocarcinoma), HuH-7 (human hepatocellular carcinoma), and ISE6 (Ixodes scapularis tick embryo) cells were found to be susceptible to IWVV infection. Notably, several human-derived cell lines supported efficient replication of the virus. Our findings provide new insights into the diversity, evolutionary dynamics, and geographical distribution of orthonairoviruses in East Asia.
Competing Interests: Declarations. Conflict of interest: The authors declare that they have no conflict of interest. Ethical approval: This study does not contain any experiments with human participants of animals.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: