Comparison of novel native probiotics and paraprobiotics in modulating oxidative stress and inflammation in DSS-induced colitis: implications for enhanced therapeutic strategies in high fat diet.

Autor: Rezaie N; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran., Aghamohammad S; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran., Gholizadeh Khiavi EHA; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran., Pourshafie MR; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran., Talebi M; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran., Rohani M; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran. m_rohani@pasteur.ac.ir.
Jazyk: angličtina
Zdroj: BMC immunology [BMC Immunol] 2024 Dec 20; Vol. 25 (1), pp. 85. Date of Electronic Publication: 2024 Dec 20.
DOI: 10.1186/s12865-024-00678-0
Abstrakt: Aim: IBD is a condition that may result from the presence of oxidative stress. The objective of this research is to evaluate and compare the potency of probiotics and paraprobiotics to modulate oxidative stress and inflammation.
Methods and Results: In the initial phase, the antioxidant capabilities of 88 strains from Lactobacillus and Bifidobacterium were evaluated. In the subsequent phase, during the in-vivo stage, four experimental groups were established, consisting of a high-fat diet (HFD) + PBS, HFD + DSS, HFD + DSS + 10^ 9 cfu/ml of 6 selected native probiotic, and HFD + DSS + 10^ 9 cfu/ml of paraprobiotic (from 6 selected strains), with male wild-type C57BL/6 mice being assigned to these groups. The phenotypical indices and pathological scores along with the evaluation of the expression of genes associated with the NF-kB and Nrf2 signaling pathways, as well as enzymes linked to oxidant/anti-oxidant activities, and proinflammatory/inflammatory cytokines were performed. A significant difference was noted among the groups exposed to DSS and groups that given our native agents. The mice receiving a blend of probiotics and paraprobiotics alongside DSS demonstrated a mitigation of the harmful impacts caused by DSS, both regarding phenotypic traits, including pathological scores and also the level of cytokines and antioxidant markers and also molecular indicators like the Nrf2 and NF-kB associated genes. Also, there was no notable difference between our native probiotic and paraprobiotic.
Conclusion: The study's findings provide evidence that the expression of inflammation can be successfully alleviated by utilizing our native probiotics and paraprobiotics, with a greater emphasis on the latter due to its inherent safety.
Impact Statements: This study highlighted the anti-inflammatory and antioxidant properties of probiotic and paraprobiotic that could be useful for patients with inflammatory status.
Competing Interests: Declarations. Ethics approval and consent to participate: This study was conducted according to the ARRIVE guidelines, the experimental protocols were established following the Declaration of Helsinki and all procedures involving animals were approved by the Animal Experimentation Committee of the Pasteur Institute of Iran (IR PII.REC1400.061) for the ethical care and use of laboratory mice. Signed informed consent was obtained from all participants and the procedures were approved by the Experimentation Committee of the Pasteur Institute of Iran (IR.PII.REC.1398.060) and Iran University of Medical Science (IR.IUMS.REC 1395.9221133201) for the ethical care. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: