TRPA1 contributes to respiratory depression from tobacco aerosol.

Autor: Chen S; Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan; Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, China., Takahashi N; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan., Okahara M; Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan., Kashiwadani H; Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan., Mori Y; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan., Hao L; Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, China., Kuwaki T; Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. Electronic address: tkuwaki@icloud.com.
Jazyk: angličtina
Zdroj: Respiratory physiology & neurobiology [Respir Physiol Neurobiol] 2024 Dec 18; Vol. 332, pp. 104385. Date of Electronic Publication: 2024 Dec 18.
DOI: 10.1016/j.resp.2024.104385
Abstrakt: Transient receptor potential ankyrin-1 (TRPA1) is expressed in the trigeminal nerves in the nasal cavity. It detects irritant chemicals such as formalin and acrolein, induces respiratory depression to protect against further inhalation, and elicits avoidance behavior. Although tobacco smoke contains formalin, acrolein, and other irritant chemicals, the possible contribution of TRPA1 to protection against tobacco smoke has yet to be fully understood. In this study, we compared respiratory and behavioral responses to an aerosol of tobacco smoke between TRPA1 conditional knockout mice and the controls. We also compared the effect of aerosols from the smoke of traditional standard tobacco and a recently developed heated tobacco product. As expected, respiratory depression by tobacco aerosol was observed only in the TRPA1 intact mice and was associated with increased trigeminal activation. Meanwhile, mice did not avoid or even prefer tobacco aerosol in a TRPA1-independent manner, contrary to our expectations. Repeated exposure to tobacco aerosol resulted in lung inflammation in a TRPA1-independent manner. Aerosols from a heated tobacco product showed no significant effect as in traditional tobacco smoke. These results indicate that TRPA1 contributes to acute protection from tobacco smoke by inducing respiratory depression but not to the safety of the lungs in repeated exposure. Tobacco aerosol contains attractive substances for mice. Heated tobacco product aerosol contains less TRPA1 activating substances and less inflammation evoking than traditional tobacco smoke.
Competing Interests: Declaration of Competing Interest The authors declare no competing interests.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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