Protective effect of oleuropein on the brain tissue in D-Galactose-induced aging in rat model.

Autor: Hu X; Department of Neurology, Shandong Public Health Clinical Center, Shandong University, Jinan, 250100, China., Zhao N; Department of Neurosurgery, The First Hospital of Kunming (Affiliated Calmette Hospital of Kunming Medical University), Kunming, 650224, China., Ranjbar E; Department of Biochemistry, Medicine School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ranjbar.elham57@gmail.com., Foruozandeh H; Blood Transfusion Research Center, High Institute for Education and Research in Transfusion Medicine, Shiraz, Iran., Nahal AS; Department of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran., Asadoola Y; Department of Nursing, Kut University College, Wasit, 52001, Iraq., Ahmadi I; Department of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran. ahmadiiraj57@yahoo.com.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Dec 20; Vol. 52 (1), pp. 67. Date of Electronic Publication: 2024 Dec 20.
DOI: 10.1007/s11033-024-10165-9
Abstrakt: Background: Oleuropein (OLE) has the potential to reduce oxidative stress and inflammation. So, in the present investigation, we explored the protective effect of OLE on brain aging induced by d-galactose (D-Gal) in a rat model.
Methods and Results: 40 Wister male adult rats were categorized into 5 groups. Group 1 received normal saline; group 2 was given 100 mg/kg of D-Gal intraperitoneally (IP). The rats in groups 3 to 5 were given D-Gal (100 mg/kg, IP) along with different doses of OLE (20, 40, and 80 mg/kg, respectively) orally. All administrations were performed daily for 8 weeks. 24 h after last treatment motor activity and memory impairment were evaluated. Then, the rats were euthanized and brain samples were collected for evaluating the levels of malondialdehyde (MDA), Brain-Derived Neurotrophic Factor (BDNF), protein carbonyl (PC), glutathione (GSH), glutathione peroxidase (GPX), catalase (CAT), Superoxide dismutase (SOD), Tumor necrosis factor alpha (TNF-α), interleukin 1 beta ( IL-1β), as well as Sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1) gene expression. The results showed that D-Gal significantly reduced motor activity and memory performance (P < 0.05). It also significantly reduced the GPX, CAT and SOD activities, GSH and BDNF levels as well as SIRT1 and PGC1 expression, and, significantly increased PC, MDA TNF-α and IL-1β levels in the brain tissue (P < 0.05). Administration of OLE restored all of the above parameters close to control group.
Conclusion: The findings demonstrated that OLE, through its antioxidant and anti-inflammatory properties, improved motor activity, memory impairment, and age-related neurological dysfunction.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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