CTFFIND5 provides improved insight into quality, tilt, and thickness of TEM samples.

Autor:	Elferich J; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, United States.; Howard Hughes Medical Institute, University of Massachusetts Chan Medical School, Worcester, United States., Kong L; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, United States., Zottig X; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, United States.; Howard Hughes Medical Institute, University of Massachusetts Chan Medical School, Worcester, United States., Grigorieff N; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, United States.; Howard Hughes Medical Institute, University of Massachusetts Chan Medical School, Worcester, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2024 Dec 20; Vol. 13. Date of Electronic Publication: 2024 Dec 20.
DOI:	10.7554/eLife.97227
Abstrakt:	Images taken by transmission electron microscopes are usually affected by lens aberrations and image defocus, among other factors. These distortions can be modeled in reciprocal space using the contrast transfer function (CTF). Accurate estimation and correction of the CTF is essential for restoring the high-resolution signal in cryogenic electron microscopy (cryoEM). Previously, we described the implementation of algorithms for this task in the cis TEM software package (Grant et al., 2018). Here we show that taking sample characteristics, such as thickness and tilt, into account can improve CTF estimation. This is particularly important when imaging cellular samples, where measurement of sample thickness and geometry derived from accurate modeling of the Thon ring pattern helps judging the quality of the sample. This improved CTF estimation has been implemented in CTFFIND5, a new version of the cis TEM program CTFFIND. We evaluated the accuracy of these estimates using images of tilted aquaporin crystals and eukaryotic cells thinned by focused ion beam milling. We estimate that with micrographs of sufficient quality CTFFIND5 can measure sample tilt with an accuracy of 3° and sample thickness with an accuracy of 5 nm. Competing Interests: JE, LK, XZ No competing interests declared, NG Reviewing editor, eLife (© 2024, Elferich et al.)
Databáze:	MEDLINE
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