Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation.

Autor:	Corval ARDC; Postgraduate Program in Veterinary Sciences, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., de Carvalho LAL; Laboratory of Bioinformatics, Department of Agricultural, Livestock and Environmental Biotechnology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.; Graduate Program in Agricultural and Livestock Microbiology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil., Mesquita E; Postgraduate Program in Veterinary Sciences, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Fiorotti J; Department of Biochemistry and Immunology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil., Corrêa TA; Postgraduate Program in Veterinary Sciences, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Bório VS; Postgraduate Program in Veterinary Sciences, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Carneiro ADS; Postgraduate Program in Veterinary Sciences, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Pinheiro DG; Laboratory of Bioinformatics, Department of Agricultural, Livestock and Environmental Biotechnology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil., Coelho IDS; Department of Veterinary Microbiology and Immunology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Santos HA; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Fernandes EKK; Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil., Angelo IDC; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Bittencourt VREP; Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Golo PS; Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Dec 05; Vol. 15, pp. 1507931. Date of Electronic Publication: 2024 Dec 05 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1507931
Abstrakt:	Metarhizium is widely known for its role as an arthropod biocontrol agent and plant bioinoculant. By using mass-production industrial methods, it is possible to produce large amounts of fungal single-celled propagules (including blastospores) to be applied in the field. However, in the environment, the solar ultraviolet components (particularly UV-B) can harm the fungus, negatively impacting its pathogenicity toward the arthropod pest. The present study is the first to use comparative genome-wide transcriptome analyses to unveil changes in gene expression between Metarhizium pingshaense blastospores exposed or not to UV-B. Relative blastospores culturability was calculated 72 h after UV-B exposure and exhibited 100% culturability. In total, 6.57% ( n = 728) out of 11,076 predicted genes in M. pingshaense were differentially expressed after UV-B exposure: 320 genes (44%; 320/728) were upregulated and 408 (56%; 408/720) were downregulated in the UV-B exposed blastospores. Results unveiled differentially expressed gene sets related to fungal virulence, production of secondary metabolites, and DNA repair associated with UV damage; genes related to virulence factors were downregulated, and genes associated with nucleotide excision repair were upregulated. These findings illustrate critical aspects of Metarhizium blastospores strategies to overcome UV-B damage and survive solar radiation exposures in insulated fields. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Corval, Carvalho, Mesquita, Fiorotti, Corrêa, Bório, Carneiro, Pinheiro, Coelho, Santos, Fernandes, Angelo, Bittencourt and Golo.)
Databáze:	MEDLINE
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