Single-cell dissection of multifocal bladder cancer reveals malignant and immune cells variation between primary and recurrent tumor lesions.

Autor: Liu S; Department of Urology, Huashan Hospital, Fudan University, 200040, Shanghai, China. liushenghuafy@163.com., Feng C; Department of Urology, Huashan Hospital, Fudan University, 200040, Shanghai, China., Tan L; Department of Urology, Huashan Hospital, Fudan University, 200040, Shanghai, China., Zhang D; Department of Chemistry and The Swire Institute of Marine Science, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China., Li YX; Department of Chemistry and The Swire Institute of Marine Science, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China., Han Y; Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital, School of Life Sciences and Technology, Tongji University, 200092, Shanghai, China. hanyaya@tongji.edu.cn.; Frontier Science Center for Stem Cells, School of Life Sciences and Technology, Tongji University, 200092, Shanghai, China. hanyaya@tongji.edu.cn., Wang C; Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital, School of Life Sciences and Technology, Tongji University, 200092, Shanghai, China. 08chenfeiwang@tongji.edu.cn.; Frontier Science Center for Stem Cells, School of Life Sciences and Technology, Tongji University, 200092, Shanghai, China. 08chenfeiwang@tongji.edu.cn.; National Key Laboratory of Autonomous Intelligent Unmanned Systems, Tongji University, 200120, Shanghai, China. 08chenfeiwang@tongji.edu.cn.; Frontier Science Center for Intelligent Autonomous Systems, Tongji University, 200120, Shanghai, China. 08chenfeiwang@tongji.edu.cn.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Dec 19; Vol. 7 (1), pp. 1659. Date of Electronic Publication: 2024 Dec 19.
DOI: 10.1038/s42003-024-07343-7
Abstrakt: Bladder carcinoma (BLCA) is characterized by a high rate of post-surgery recurrence and multifocality. Multifocal tumors have a higher risk of recurrence compared to single tumors, significantly impacting bladder cancer-specific mortality. However, the interregional or intraregional heterogeneity within both primary and recurrent tumors remains poorly understood. Here, we employed single-cell RNA sequencing to analyze tumor lesions from five multifocal bladder cancer patients comprising three primary tumors and two recurrent tumors. Our findings revealed that malignant cells derived from recurrent multifocal bladder cancer exhibited higher interregional transcriptional similarity and consistent cellular communication. Furthermore, our analysis uncovered that malignant cells from recurrent tumors may evade immune destruction by suppressing cytokine responses and natural killer cell activity. Notably, we identified a preference for the expression of the tryptophan metabolic enzyme IL4I1 on SPP1 + macrophages in recurrent tumors. Functional analyses have revealed that IL4I1 may promotes tumor progression in recurrent tumors by activating the aryl hydrocarbon receptor (AHR) and recruiting regulatory T cells to suppress adaptive immunity. Taken together, our study provides a comprehensive understanding of primary and recurrent multifocal bladder tumors, offering valuable resources for analyzing the multifocality and recurrence of bladder cancer.
Competing Interests: Competing interests: The authors declare no competing interests. Ethics approval and consent to participate: Informed consent was obtained for all patients and the study was approved by Shanghai Tenth People’s Hospital (22KN209).
(© 2024. The Author(s).)
Databáze: MEDLINE
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