A combined therapy of meropenem-ZnO nanoparticles efficiently eliminates carbapenem-resistant Klebsiella pneumoniae biofilms, with reduced nephrotoxicity (in vitro).

Autor:	Mohamed AA; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt.; Graduate School of Natural and Applied Science, Ege University, Izmir, Türkiye., Saed S; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt., El-Sayed SR; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt., Yassin MT; Botany and Microbiology Department, College of Science, King Saud University, 2455 Riyadh, Saudi Arabia.; King Salman Center for Disability Research, Riyadh 11614, Saudi Arabia., Gad M; Zoology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt., Tartour E; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt., Fathey HA; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt., Taha AS; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt., Mohamed AH; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt., Al-Otibi FO; Botany and Microbiology Department, College of Science, King Saud University, 2455 Riyadh, Saudi Arabia., AbdelGawwad MR; Genetics and Bioengineering Department, Faculty of Engineering and Natural Sciences, International University of Sarajevo, 71210 Sarajevo, Bosnia and Herzegovina., Ahmed MMS; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt., Almalki SA; Laboratory medicine department, Faculty of Applied Medical Sciences, Al-Baha University, 65779, Saudi Arabia., Abdel-Haleem M; Microbiology and Botany Department, Faculty of Science, Zagazig University,Zagazig 44519, Egypt.
Jazyk:	angličtina
Zdroj:	Letters in applied microbiology [Lett Appl Microbiol] 2024 Dec 02; Vol. 77 (12).
DOI:	10.1093/lambio/ovae136
Abstrakt:	In response to the World Health Organization's research agenda of antimicrobial resistance in human health, this study appraised the antibacterial and antibiofilm synergistic activity of meropenem and ZnO nanoparticles (ZnO-NPs) combination against carbapenem-resistant Klebsiella pneumoniae (CRKP). The minimum inhibitory concentration (MIC) of meropenem in combination was found to be ~1/12 of its MIC alone. The results of microtiter dilution assay showed that the combination was more efficient in reducing the biofilm biomass than meropenem alone or ZnO-NPs alone. The scanning-electron-microscopy micrographs elucidated that the combination of meropenem with ZnO-NPs has significantly enhanced its competence in eradicating the preformed biofilms of CRKP strains. In addition, the relative gene expression results showed that the combination compared to the meropenem alone and ZnO-NPs alone eloquently down-regulated the expression of biofilm genes (mrkA, fimA, and ecpA). Besides, the MTT-assay demonstrated that the combination has limited cytotoxicity against Vero-cells (in vitro). Overall, this study represents an efficient safe enhancement of meropenem to tackle the growing health threat of CRKP and carbapenem-resistant Enterobacterals prevalence. (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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