| Autor: |
Liu WZ; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China., Zhou SW; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China., Zhang SK; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China., Wang LM; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China., Gu XP; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China., Chu LX; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China., Qiao L; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China., Wan J; the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450046, China., Zhang X; Xinyang Lingrui Ecological Agriculture Co., Ltd. Xinyang 460000, China., Yang LL; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province Henan Zhengzhou 450046, China Engineering and Technology Center for Chinese Medicine Development of Henan Province Zhengzhou 450046, China., Dong CM; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province Henan Zhengzhou 450046, China Engineering and Technology Center for Chinese Medicine Development of Henan Province Zhengzhou 450046, China., Feng WS; School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province Henan Zhengzhou 450046, China Engineering and Technology Center for Chinese Medicine Development of Henan Province Zhengzhou 450046, China. |
| Abstrakt: |
Based on whole genome data, the identification and expression pattern analysis of the Atropa belladonna WRKY transcription factor family were conducted to provide a theoretical foundation for studying the biological functions and mechanisms of these transcription factors. In this study, bioinformatics methods were employed to identify members of the A. belladonna WRKY gene family and to predict their physicochemical properties, conserved motifs, promoter cis-acting elements, and chromosomal localization. Additionally, the expression patterns of the A. belladonna WRKY gene family under the regulation of environmental factors such as light quality and temperature were analyzed. The results revealed a total of 28 AbWRKY transcription factors, randomly distributed across 16 chromosomes, encoding 324-707 amino acids. Most AbWRKY proteins were acidic, unstable, and hydrophilic. Based on multiple sequence alignment and phylogenetic analysis, the WRKY gene family members were classified into two subfamilies. Conserved motif and domain analysis indicated that WRKY transcription factors in the same subfamily possessed conserved structural features. Promoter analysis predicted that the A. belladonna WRKY family contained light-responsive elements, hormone-responsive elements, and stress-responsive elements. Collinearity analysis showed that AbWRKY24 plays a crucial role in the expansion of the AbWRKY gene family. Then qRT-PCR results indicated that AbWRKY6, AbWRKY8, AbWRKY14, and AbWRKY24 responded to red light stress, while AbWRKY8, AbWRKY14, and AbWRKY24 responded to yellow light/low-temperature combined stress. AbWRKY6 and AbWRKY8 were significantly expressed in leaves and stems, AbWRKY27 and AbWRKY28 were significantly expressed in fibrous roots, and AbWRKY25 was significantly expressed in flowers. This study is the first to identify and analyze the WRKY gene family in A. belladonna and to examine its expression patterns under light and temperature regulation, laying a foundation for in-depth analysis and functional validation of the molecular mechanisms of A. belladonna WRKY transcription factors in responding to light quality and temperature environmental factors. |
