Effect of time and temperature on the stability of HPV and cellular nucleic acid using simulated dry self-samples.

Autor: Connor L; Scottish HPV Reference Laboratory, NHS Lothian, Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, United Kingdom. Electronic address: linzi.connor@nhslothian.scot.nhs.uk., Davey A; HPV Research Group, University of Edinburgh, Edinburgh EH16 4TJ, United Kingdom., Danial J; Scottish HPV Reference Laboratory, NHS Lothian, Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, United Kingdom., Moncur S; HPV Research Group, University of Edinburgh, Edinburgh EH16 4TJ, United Kingdom., Elasifer H; HPV Research Group, University of Edinburgh, Edinburgh EH16 4TJ, United Kingdom., Graham C; Edinburgh Clinical Research Facility, University of Edinburgh, Scotland EH4 2XU, United Kingdom., Cuschieri K; Scottish HPV Reference Laboratory, NHS Lothian, Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, United Kingdom; HPV Research Group, University of Edinburgh, Edinburgh EH16 4TJ, United Kingdom.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2024 Dec 17; Vol. 333, pp. 115101. Date of Electronic Publication: 2024 Dec 17.
DOI: 10.1016/j.jviromet.2024.115101
Abstrakt: Background: Self-sampling is now a key component within HPV-based cervical screening programmes to engage individuals and enhance participation. As self-sampling is relatively new, information on the influence of pre-analytical parameters such as transit-temperature and time between sampling and testing on HPV test results requires detailed investigation.
Methods: FLOQSwabs® and Evalyn Brushes® were used to assess HPV and cellular stability over a 30-week period (0w,4w,12w,30w) at 4 °C, ambient, and 37 °C. Vaginal self-samples were simulated by inoculating the devices with an HPV16-positive cell-line suspension. Devices were tested using two DNA-based (Anyplex™ II HPV28, Papilloplex® HR-HPV), one mRNA-based (APTIMA HR-HPV,) and one in-house beta-globin qPCR assay.
Results: No loss of qualitative HPV detection was observed after 12-weeks storage at ambient or 4°C irrespective of device or assay. For DNA-based assays, no loss of qualitative HPV detection was observed over time (30w) irrespective of temperature/device. Loss of qualitative mRNA signal was observed when devices were stored at 37°C for 12-weeks or longer; however, no loss of detection was observed at 30-weeks when either device was stored at 4°C.
Conclusion: HPV nucleic acid is stable on proxies of self-taken samples, however, the duration of stability was affected by the device and storage conditions. Such differences should be considered when optimising self-sampling exercises.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: LC, AD, JD, SM, HE, CG, KC’s institution received research funding, free-of-charge reagents, and consumables to support research in the last three years from Abbott, Euroimmun, GeneFirst, SelfScreen, Hiantis, Seegene, Roche, Hologic, Barinthus Biotherapeutics PLC & Daye. KC has attended advisory board meetings for Hologic and Barinthus Biotherapeutics PLC., all paid to their employer.
(Crown Copyright © 2024. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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