Absolute quantification of Neuron-specific enolase based on surface plasmon resonance.

Autor:	Lin C; Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, 310018, China., Wang Y; Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, 310018, China., Peng T; Center for Advanced Measurement Science, Technology Innovation Center of Mass Spectrometry for State Market Regulation, National Institute of Metrology, Beijing, 100029, China., Liu P; Zhejiang Fangyuan Test Group Co., Ltd, Hangzhou, 310018, China; Key Laboratory of Biosafety detection for Zhejiang Market Regulation, Hangzhou, 310018, China., Liang Y; Zhejiang Fangyuan Test Group Co., Ltd, Hangzhou, 310018, China; Key Laboratory of Biosafety detection for Zhejiang Market Regulation, Hangzhou, 310018, China., Kang W; Inner Mongolia Autonomous Region Institute of Metrology and Testing, Inner Mongolia Autonomous Region, 010050, China., Yu X; Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, 310018, China., Song Y; Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, 310018, China. Electronic address: songyang@cjlu.edu.cn., Shentu X; Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, 310018, China. Electronic address: stxp@cjlu.edu.cn.
Jazyk:	angličtina
Zdroj:	SLAS discovery : advancing life sciences R & D [SLAS Discov] 2024 Dec 17; Vol. 30, pp. 100205. Date of Electronic Publication: 2024 Dec 17.
DOI:	10.1016/j.slasd.2024.100205
Abstrakt:	Neuron-specific enolase (NSE) is currently the most reliable biomarker for small cell lung cancer (SCLC), which is important for disease monitoring, clinical evaluation and diagnosis. However, traditional methods suffer from various disadvantages, including instability, complexity, time-consuming operations, and the necessity for standards. In this study, we developed a calibration-free concentration analysis (CFCA) method based on surface plasmon resonance (SPR) technology, to accurately quantify the active concentration of NSE without relying on any standards. Based on the principle of CFCA, the active concentration of NSE can be calculated by observing binding rate variations at two flow rates under partial mass transport limitation and combining it with the known diffusion coefficient of the NSE. Using the method of CFCA, the active concentration of NSE was determined was only 0.48 mg/mL with an intra-day repeatability of 4.75%. The method has the advantages of simplicity, rapidity, realistic analysis and ease of implementation of high-throughput automated detection. Therefore, the method is expected to become the main measurement method for protein active concentration, which will be beneficial for the development of active protein standards. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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