Modification of a O-acetyl-glucomannan from Dendrobium officinale by selenylation modification and its anti-gastric cancer enhancing activity.

Autor: Liu J; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China., Yan D; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China., Chen Q; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China., Peng H; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China., Jin L; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China., Feng C; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China., Zhang L; Yunnan Branch, Institute of Medicinal Plant, Chinese Academyl of Medical Sciences, Peking Union Medical College, Jinghong, Yunnan 666100, China., Zhao G; Guangdong L-Med Biotechnology Co., Lt, Guangzhou, Guangdong 510530, China., Yang D; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China., Zhao Z; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China. Electronic address: zhaozhm2@mail.sysu.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec 17, pp. 138852. Date of Electronic Publication: 2024 Dec 17.
DOI: 10.1016/j.ijbiomac.2024.138852
Abstrakt: In this study, a homogeneous polysaccharide, named YDOP-1 was isolated form Dendrobium officinale using hot water extraction and ethanol precipitation method. YDOP-1 was characterized to be a typical O-acetyl-glucomannan with the molecular wight was 13,456 Da. Cell viability and colony forming assay showed that YDOP-1 possess moderate anti-gastric cancer effects. In order to further improve the anti-gastric cancer effects of YDOP-1, a selenium modification on YDOP-1 was performed. Energy dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Se was successfully introduced into YDOP-1 by forming SeO bonds. Se modification significantly enhanced the anti-gastric cancer effects of YDOP-1, which could effectively inhibit the proliferation of MGC-803 cells via suppressing colony and inducing apoptosis by regulating the apoptosis proteins of Bax, Bcl-2, and Caspase-7. Our results indicated that the Se modified O-acetyl-glucomannan, YDOP-Se, was expected to be useful in the biomedicine field for the gastric cancer treatment.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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