In-vivo Raman microspectroscopy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots.
Autor: | Fernández González A; Department of Soil and Crop Sciences, Texas A&M University and Texas A&M AgriLife Research, College Station, TX, USA.; Institute of Quantum Science and Engineering, Texas A&M University, College Station, TX, USA., Fang ZT; Department of Soil and Crop Sciences, Texas A&M University and Texas A&M AgriLife Research, College Station, TX, USA., Sen D; Institute of Quantum Science and Engineering, Texas A&M University, College Station, TX, USA.; Department of Physics and Astronomy, Texas A&M University, College Station, TX, USA., Henrich B; Department of Soil and Crop Sciences, Texas A&M University and Texas A&M AgriLife Research, College Station, TX, USA., Nagashima Y; Department of Horticultural Sciences, Texas A&M University and Texas A&M AgriLife Research, College Station, TX, USA., Sokolov AV; Institute of Quantum Science and Engineering, Texas A&M University, College Station, TX, USA.; Department of Physics and Astronomy, Texas A&M University, College Station, TX, USA., Okumoto S; Department of Soil and Crop Sciences, Texas A&M University and Texas A&M AgriLife Research, College Station, TX, USA. Sakiko.Okumoto@ag.tamu.edu., Verhoef AJ; Department of Soil and Crop Sciences, Texas A&M University and Texas A&M AgriLife Research, College Station, TX, USA. Aart.Verhoef@ag.tamu.edu.; Institute of Quantum Science and Engineering, Texas A&M University, College Station, TX, USA. Aart.Verhoef@ag.tamu.edu.; Department of Physics and Astronomy, Texas A&M University, College Station, TX, USA. Aart.Verhoef@ag.tamu.edu. |
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Jazyk: | angličtina |
Zdroj: | Plant methods [Plant Methods] 2024 Dec 18; Vol. 20 (1), pp. 185. Date of Electronic Publication: 2024 Dec 18. |
DOI: | 10.1186/s13007-024-01302-3 |
Abstrakt: | Background: Nitrate (NO Results: As a proof of concept, we have used Raman microspectroscopy for in-vivo mapping of nitrate content in roots of Arabidopsis seedlings grown on agar media with different nitrate concentrations. Our results revealed that the root nitrate concentration increases gradually from the meristematic zone (~ 250 µm from the root cap) to the maturation zone (~ 3 mm from the root cap) in roots grown under typical growth conditions used for Arabidopsis, a trend that has not been previously reported. This trend was observed for plants grown in agar media with different nitrate concentrations (0.5-10 mM). These results were validated through destructive measurement of nitrate concentration. Conclusions: We present a methodology based on Raman microspectroscopy for in-vivo label-free mapping of nitrate within small root tissue volumes in Arabidopsis. Measurements are done in-situ without additional sample preparation. Our measurements revealed nitrate concentration changes from lower to higher concentration from tip to mature root tissue. Accumulation of nitrate in the maturation zone tissue shows a saturation behavior. The presented Raman-based approach allows for in-situ non-destructive measurements of Raman-active compounds. Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. (© 2024. The Author(s).) |
Databáze: | MEDLINE |
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